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Inflammatory changes in the choroid plexus following subarachnoid hemorrhage: the role of innate immune receptors and inflammatory molecules.
Front Cell Neurosci
January 2025
Department of Anatomy, Faculty of Medicine, Masaryk University, Brno, Czechia.
Introduction: The choroid plexus is located in the cerebral ventricles. It consists of a stromal core and a single layer of cuboidal epithelial cells that forms the blood-cerebrospinal barrier. The main function of the choroid plexus is to produce cerebrospinal fluid.
View Article and Find Full Text PDFDevelopment of an H&E on-block staining technique for collagen detection in cryo-fluorescence tomography imaging of frozen breast tissue samples.
medRxiv
December 2024
Department of Imaging Physics, The University of Texas MD Anderson Cancer Center, Houston, TX.
Background: Hematoxylin and eosin (H&E) staining is widely considered to be the gold-standard diagnostic tool for histopathology evaluation. However, the fatty nature of some tissue types, such as breast tissue, presents challenges with cryo-sectioning, often resulting in artifacts that can make histopathologic interpretation and correlation with other imaging modalities virtually impossible. We present an optimized on-block H&E staining technique that improves contrast for identifying collagenous stroma during cryo-fluorescence tomography (CFT) sectioning.
View Article and Find Full Text PDFInflammatory and degenerative changes in the extensor pollicis longus muscle and tendon following ruptures caused by distal radius fractures.
BMC Musculoskelet Disord
January 2025
Department of Hand Surgery, Sahlgrenska University Hospital, Gothenburg, Sweden.
Background: Rupture of extensor pollicis longus tendon (EPL) is a known complication following a distal radius fracture (DRF). Although the precise mechanisms behind these ruptures remain unclear, vascular impairment is thought to play a significant role. Additionally, the impact of an EPL rupture on microstructure of the tendon and muscle is not well understood, but such information could be important in guiding treatment strategies.
View Article and Find Full Text PDFUse of polyethylene glycol as an alternative to optimal cutting temperature medium in freeze sectioning for plant histochemical studies.
Protoplasma
December 2024
Natural Product Biotechnology Group, Agricultural and Food Engineering Department, Indian Institute of Technology Kharagpur, Kharagpur, 721 302, India.
Plant anatomical and histochemical studies are concerned with the structural organization of tissues as well as localization of various metabolites and enzyme activity inside cells and tissues. Traditionally, rotary microtomes are used for paraffin and resin-embedded samples which provide excellent preservation of tissue morphology but removes enzymes, lipid components, and various specialized metabolites. Freeze sectioning apparently remained unexplored in plant histology because of the presence of rigid cell walls and highly vacuolated cytoplasm in plant tissues.
View Article and Find Full Text PDFEvaluation of dendrite morphology in Wistar and genetic absence epileptic rats.
Brain Struct Funct
December 2024
Department of Anatomy, School of Medicine, Marmara University, Istanbul, Turkey.
Objective: Genetic Absence Epilepsy Rat from Strasbourg (GAERS), a rodent model genetically predisposed to absence epilepsy, serves as an experimental tool to elucidate the neuronal mechanisms underlying human absence epilepsy. This study aimed to investigate the morphological features of dendrites and dendritic spines of pyramidal neurons in somatosensory cortex and hippocampus of Wistar and GAERS rats.
Material And Method: Adult male GAERS (n = 5) and control Wistar (n = 5) rats were sacrificed by transcardial perfusion and brains were removed.
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