AI Article Synopsis
- Pyridoxal kinase is an important enzyme that converts vitamin B(6) to its active form, pyridoxal-5'-phosphate, which is essential for amino acid metabolism and neurotransmitter synthesis.
- The enzyme was successfully crystallized from sheep brain using the hanging-drop vapor-diffusion technique and sodium citrate as a precipitant.
- The resulting crystals are of high quality, belonging to a specific space group and capable of diffracting at a resolution of 2.1 A, and were further treated to prepare heavy-atom derivatives for analysis.
Article Abstract
Pyridoxal kinase (ATP:pyridoxal 5'-phosphotransferase; EC 2.7.1.35) is a key enzyme in the transformation of vitamin B(6) to pyridoxal-5'-phosphate. Pyridoxal-5'-phosphate is the crucial cofactor required by numerous enzymes involved in the metabolism of amino acids and the synthesis of many neurotransmitters. Pyridoxal kinase from sheep brain was crystallized in an orthorhombic form using the hanging-drop vapour-diffusion method with sodium citrate as the precipitant. The crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 59.8, b = 94.4, c = 128.2 A, and diffract to a resolution of 2.1 A. Crystals were transferred into a soaking liquid without citrate and two heavy-atom derivatives were prepared.
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| http://dx.doi.org/10.1107/S0907444902011034 | DOI Listing |
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