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Chemokine profile in the serum of patients with leptospirosis.
Front Cell Infect Microbiol
November 2024
Laboratory of Bacteriology, Butantan Institute, Sao Paulo, Brazil.
Introduction: Leptospirosis is a global zoonosis that affects more than one million people per year, with a lethality rate of approximately 15%. Chemokines are crucial in the immune response against , recruiting leukocytes to the site of infection and regulating immune activity. In previous studies, we have shown that CCL2, CXCL5, and CCL8 are involved in the leptospirosis process, although the mechanisms are not understood.
View Article and Find Full Text PDFCRISPR-prime editing, a versatile genetic tool to create specific mutations with a single nucleotide resolution in .
mBio
September 2024
Infectious Bacterial Disease Research Unit, USDA Agricultural Research Service, National Animal Disease Center, Ames, Iowa, USA.
Leptospirosis, caused by pathogenic bacteria from the genus , is a global zoonosis responsible for more than one million human cases and 60,000 deaths annually. The disease also affects many domestic animal species. Historically, genetic manipulation of has been difficult to perform, resulting in limited knowledge on pathogenic mechanisms of disease and the identification of virulence factors.
View Article and Find Full Text PDFLipL41 and LigA/LigB Gene Silencing on a LipL32 Knockout Reveals the Impact of Multiple Mutations on Virulence.
Pathogens
September 2023
Laboratório de Desenvolvimento de Vacinas, Instituto Butantan, Avenida Vital Brazil, 1500, São Paulo 05503-900, SP, Brazil.
Leptospirosis is a global zoonosis caused by pathogenic bacteria of the genus . The application of the CRISPR/Cas9 system has facilitated the generation of mutants and subsequent evaluation of phenotypes. Since DNA breaks induced by RNA-guided Cas9 nuclease are lethal to , different methodologies were implemented to overcome this limitation.
View Article and Find Full Text PDFEvaluation of knockdown mutants for LipL32, LipL41, LipL21, and OmpL1 proteins.
Front Microbiol
June 2023
Laboratório de Desenvolvimento de Vacinas, Instituto Butantan, São Paulo, SP, Brazil.
Introduction: Leptospirosis is a worldwide zoonosis caused by pathogenic and virulent species of the genus , whose pathophysiology and virulence factors remain widely unexplored. Recently, the application of CRISPR interference (CRISPRi) has allowed the specific and rapid gene silencing of major leptospiral proteins, favoring the elucidation of their role in bacterial basic biology, host-pathogen interaction and virulence. Episomally expressed dead Cas9 from the CRISPR/Cas system (dCas9) and single-guide RNA recognize and block transcription of the target gene by base pairing, dictated by the sequence contained in the 5' 20-nt sequence of the sgRNA.
View Article and Find Full Text PDFSocio-geographical factors and vulnerability to leptospirosis in South Brazil.
BMC Public Health
July 2023
Department of Preventive Veterinary Medicine, Federal University of Pelotas, Capão Do Leão, Rio Grande Do Sul, Brazil.
Background: Leptospirosis, caused by the Leptospira bacteria, is an acute infectious disease that is mainly transmitted by exposure to contaminated soil or water, thereby presenting a wide range of subsequent clinical conditions. This study aimed to assess the distribution of cases and deaths from leptospirosis and its association with social vulnerability in the state of Rio Grande do Sul, Brazil, between 2010 and 2019.
Methods: The lethality rates and incidence of leptospirosis and their association with gender, age, education, and skin color were analyzed using chi-square tests.
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