AI Article Synopsis
- - A new direct competitive ELISA method was developed for detecting polychlorinated biphenyls (PCBs) in soil, using Delor 103 as a standard, with a calibration range of 10-1000 µg/L and a detection limit of 5.0-12.9 µg/L.
- - Soil samples spiked with PCBs showed recovery rates of 90.6-106.3% when extracted with methanol, and the effects of methanol and DMSO on assay performance were evaluated.
- - When comparing results from the ELISA and gas chromatography (GC) on eight PCB-contaminated soil samples, the ELISA showed high correlation with GC results, with the exception of
Article Abstract
A direct competitive ELISA for determination of polychlorinated biphenyls (PCB) in soil samples was described. The standard calibration curve based on Delor 103 (Aroclor 1242) was constructed in the dynamic range of 10-1000 micro g L(-1) and a detection limit of 5.0-12.9 micro g L(-1) (or 0.5-1.29 micro g g(-1) soil) was achieved. When spiked soil samples were extracted with methanol recoveries were 90.6-106.3 %. The effect of methanol and DMSO on assay signal and sensitivity was established. Eight PCB-contaminated soil samples were analyzed by ELISA and gas chromatography (GC). The ELISA results from Soxhlet extraction were in a good agreement with those of GC (correlation coefficient 0.9866; n=8). Except for one soil sample the results from ELISA with methanol extraction were not significantly different from those from GC.
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| http://dx.doi.org/10.1007/s00216-002-1311-1 | DOI Listing |
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