AI Article Synopsis
- A specific mRNA can be synthesized from a DNA fragment using reverse transcription PCR (RT-PCR), which amplifies the concentration of the target cDNA with each cycle.
- The increase in PCR product reflects the presence of the corresponding mRNA, allowing for quantification.
- Ethidium bromide's fluorescence intensity, which enhances when bound to DNA, is measured to assess mRNA expression levels based on the PCR amplification.
Article Abstract
A DNA fragment that forms the template of a specific mRNA can be synthesized by reverse transcription PCR. In due number of cycle, the concentration of PCR product increases proportionally to that of the template cDNA, i.e., the corresponding mRNA in RT. Since the fluorescence quantum yield of ethidium bromide greatly increased when it was inserted into the DNA double helix, the extend of expression of mRNA can be calculated by determining the fluorescence intensity under selected excitation and emission wavelengths of PCR cDNA after a proper number of PCR cycle.
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