Development of a bipartite method for Fusarium identification based on cellobiohydrolase-C: CAPS and Western blot analysis.

FEMS Microbiol Lett

U.M.R. 7100, Université Louis Pasteur-CNRS, Ecole Supérieure de Biotechnologie de Strasbourg, Boulevard Sébastien Brant, 67400 Illkirch-Graffenstaden, France.

Published: August 2002

The identification of 12 Fusarium strains isolated from diseased hops (Humulus lupulus, L.) was achieved by a strategy based on cellobiohydrolase-C: cleaved amplified polymorphic sequence analysis targeting the gene and the use of an antibody directed against a peptide of the Fusarium graminearum enzyme. This strategy is shown to be rapid and reliable for all the Fusarium of our collection: F. avenaceum, F. graminearum, F. sambucinum, F. sporotrichioides, F. tricinctum and F. venenatum.

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http://dx.doi.org/10.1111/j.1574-6968.2002.tb11313.xDOI Listing

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