[2H4]Folic acid was synthesized by deuterating p-aminobenzoic acid, which was then coupled to glutamic acid and 6-formylpterin. Using [2H4]folic acid as starting component enabled the preparation of labeled vitamers tetrahydrofolate, 5-formyltetrahydrofolate, 5-methyltetrahydrofolate, and 10-formylfolate which were characterized by electrospray mass spectrometry and collision-induced dissociation. The mass spectrometric studies confirmed that the compounds could be used as internal standards in stable isotope dilution assays.

Download full-text PDF

Source
http://dx.doi.org/10.1021/jf025571kDOI Listing

Publication Analysis

Top Keywords

labeled vitamers
8
internal standards
8
standards stable
8
stable isotope
8
isotope dilution
8
dilution assays
8
[2h4]folic acid
8
acid
5
syntheses labeled
4
vitamers folic
4

Similar Publications

Brazil has a diverse plant community, including underutilized non-conventional food crops (PANCs), which have the potential to be a rich source of food and contribute to food security. For assessing the folate content in a range of Brazilian PANCs, we extended the validation of an existing stable isotope dilution assay (SIDA) for the stably C-labelled 10-formyl-Pte[C]Glu (10-CHO-Pte[C]Glu). The SIDA method with an enzymatic treatment, purification step, and an LC-MS/MS measurement was validated regarding linearity, precision, LoD/LoQ, and recovery for 10-CHO-PteGlu.

View Article and Find Full Text PDF

Climate change directs the focus in biotechnology increasingly on one-carbon metabolism for fixation of CO and CO-derived chemicals (e.g. methanol, formate) to reduce our reliance on both fossil and food-competing carbon sources.

View Article and Find Full Text PDF

Preventing Vitamin B6-Related Neurotoxicity.

Am J Ther

January 2023

Division of General Internal Medicine, UF Health Jacksonville, Jacksonville, FL.

Background: Vitamin B6 is essential for life and plays a critical role in many biochemical and physiological processes in the human body. The term B6 collectively refers to 6 water-soluble vitamers, and only the pyridoxal 5'-phosphate (PLP) serves as the biologically active form. A plasma PLP concentration above 30 nmol/L (7.

View Article and Find Full Text PDF

Validated UPLC-MS/MS method for the analysis of vitamin B pyridoxal 5́-phosphate, pyridoxal, pyridoxine, pyridoxamine, and pyridoxic acid in human cerebrospinal fluid.

J Chromatogr B Analyt Technol Biomed Life Sci

December 2022

Department of General Pediatrics, Division of Neuropediatrics and Metabolic Medicine, Center for Pediatric and Adolescent Medicine, University Hospital Heidelberg, 69120 Heidelberg, Germany.

Vitamin B and its metabolites play a crucial role in the development and interaction of brain metabolism. Following diagnostic improvements additional inherited disorders in vitamin B metabolism have been identified, most of them leading to a severe epileptic disorder accompanied by progressive neurological deficits including intellectual disability and microcephaly. Since early treatment can improve the outcome, fast and reliable detection of metabolic biomarkers is important.

View Article and Find Full Text PDF

Cobalamins (vitamin B12) are required by humans for their essential roles as enzyme cofactors in diverse metabolic processes. The four most common cobalamin vitamers are hydroxocobalamin (OHCbl), adenosylcobalamin (AdoCbl), methylcobalamin (MeCbl), and cyanocobalamin (CNCbl). Humans are not able to synthesise cobalamins and thus must acquire them from external sources.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!