Gene silencing by double-stranded RNA is a widespread phenomenon called RNAi, involving homology-dependent degradation of mRNAs. Here we show that RNAi is established in the Drosophila female germ line. mRNA transcripts are translationally quiescent at the arrested oocyte stage and are insensitive to RNAi. Upon oocyte maturation, transcripts that are translated become sensitive to degradation while untranslated transcripts remain resistant. Mutations in aubergine and spindle-E, members of the PIWI/PAZ and DE-H helicase gene families, respectively, block RNAi activation during egg maturation and perturb translation control during oogenesis, supporting a connection between gene silencing and translation in the oocyte.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC186417 | PMC |
| http://dx.doi.org/10.1101/gad.990802 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Atractylenolide Ⅲ partially alleviates tunicamycin-induced damage in porcine oocytes during in vitro maturation by reducing oxidative stress.
Anim Reprod Sci
December 2024
China-Japan Union Hospital of Jilin University, Rehabilitation Medicine Department, Changchun, Jilin, China. Electronic address:
Assisted reproductive technology (ART) is widely used to address infertility and enhance reproductive outcomes in livestock. Among various ART techniques, in vitro maturation (IVM) is commonly used to obtain high-quality oocytes but is susceptible to oxidative stress. In traditional Chinese medicine, Rhizoma Atractylodis Macrocephalae (Bai Zhu) is used to enhance maternal and fetal health.
View Article and Find Full Text PDFImpact of Amphiregulin on Oocyte Maturation and Embryo Quality: Insights from Clinical and Molecular Perspectives.
Geburtshilfe Frauenheilkd
January 2025
Department of Gynecology, Obstetrics and Reproductive Medicine, Saarland University Hospital, Homburg, Saar, Germany.
Introduction: Identifying non-invasive biomarkers which can predict the outcome of intracytoplasmic sperm injection (ICSI) is crucial, particularly in Germany where the challenges are intensified by the Embryo Protection Act. Recent research has highlighted biomarkers within the epidermal growth factor (EGF) family as central to follicular processes, although their predictive utility remains a subject of debate in the literature. Therefore, the primary objective of this study was to investigate the significance of amphiregulin concentrations in follicular fluid and gene expression in mural granulosa cells on oocyte maturation, fertilization, and embryo quality.
View Article and Find Full Text PDFEffects of maternal liver abnormality on maturation of bovine oocytes.
Zygote
January 2025
University Farm, Faculty of Agriculture, Utsunomiya University, Utsunomiya, Tochigi 321-4415, Japan.
In cattle, maternal metabolic health has been suggested to influence oocyte and embryo quality. Here, we examined whether maternal liver abnormalities affected oocyte maturation by screening meiotic maturation, spindle morphology, actin filaments, and lysosomes. In oocytes from the abnormal liver group, the maturation rate (80.
View Article and Find Full Text PDFZAR1/2-Regulated Epigenetic Modifications are Essential for Age-Associated Oocyte Quality Maintenance and Zygotic Activation.
Adv Sci (Weinh)
January 2025
Department of Obstetrics and Gynecology, Zhejiang Key Laboratory of Precise Protection and Promotion of Fertility, Zhejiang Provincial Clinical Research Center for Reproductive Health and Disease, Assisted Reproduction Unit, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, 310016, China.
The developmental competence and epigenetic progression of oocytes gradually become dysregulated with increasing maternal age. However, the mechanisms underlying age-related epigenetic regulation in oocytes remain poorly understood. Zygote arrest proteins 1 and 2 (ZAR1/2) are two maternal factors with partially redundant roles in maintaining oocyte quality, mainly known by regulating mRNA stability.
View Article and Find Full Text PDFLuteinizing hormone receptor deficiency in immature cumulus-oocyte complexes retrieved for assisted reproduction.
F S Sci
January 2025
Division of Human Reproduction, Department of Obstetrics and Gynecology, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil. Electronic address:
This study investigated whether luteinizing hormone receptor (LHR) expression varies in the granulosa cells of individual follicles according to the maturation stage of the oocytes harvested for assisted reproductive technology (ART) treatment. We observed minimal to no LHR mRNA and protein expression in cumulus cells surrounding oocytes arrested in the germinal vesicle (GV) stage. Interestingly, their ability to mature was confirmed by rescue in vitro maturation, suggesting somatic cell LHR deficiency as a key factor for the retrieval of GV oocytes in ART procedures.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!