The technique of electron microsco-pic in situ hybridization is applying in situ hybridization at the electron microscopic level. It is mainly used in the ultrastructural localization of the lablled DNA, RNA and RHA in a cell and/or a tissue. In this paper I mainly elaborated its establishment and classification, and the operation procedure of nonradioactive electron microscopic in situ hybridization and some points for attention. In the end I also discussed its application for research.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Spin State Modulation with Oxygen Vacancy Orientates C/N Intermediates for Urea Electrosynthesis of Ultrahigh Efficiency.
Adv Mater
January 2025
State Key Laboratory of Green Pesticide, Key Laboratory of Green Pesticide & Agricultural Bioengineering, Ministry of Education, State-Local Joint Laboratory for Comprehensive Utilization of Biomass, Center for R&D of Fine Chemicals, Guizhou University, Guiyang, 550025, China.
The co-electrolysis of CO and NO to synthesize urea has become an effective pathway to alternate the conventional Bosch-Meiser process, while the complexity of C-/N-containing intermediates for C-N coupling results in the urea electrosynthesis of unsatisfactory efficiency. In this work, an electronic spin state modulation maneuver with oxygen vacancies (Ov) is unveiled to effectively meliorate the oriented generation of key intermediates NH and CO for C-N coupling, furnishing urea in ultrahigh yield of 2175.47 µg mg h and Faraday efficiency of 70.
View Article and Find Full Text PDFRibosome profiling and single-cell RNA sequencing identify the unfolded protein response as a key regulator of pigeon lactation.
Zool Res
January 2025
National Key Laboratory for Swine Genetic Improvement and Germplasm Innovation, Ministry of Science and Technology of China, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China.
Pigeons and certain other avian species produce a milk-like secretion in their crop sacs to nourish offspring, yet the detailed processes involved are not fully elucidated. This study investigated the crop sacs of 225-day-old unpaired non-lactating male pigeons (MN) and males initiating lactation on the first day after incubation (ML). Using RNA sequencing, ribosome profiling, and single-cell transcriptome sequencing (scRNA-seq), we identified a significant up-regulation of genes associated with ribosome assembly and protein synthesis in ML compared to MN.
View Article and Find Full Text PDFMonocyte-Derived cxcl12 Guides a Directional Migration of Blood Vessels in Zebra Fish.
Arterioscler Thromb Vasc Biol
January 2025
School of Life Science, Nantong Laboratory of Development and Diseases and Co-Innovation Center of Neuroregeneration, Nantong University, China.
Background: Sprouting blood vessels, reaching the aimed location, and establishing the proper connections are vital for building vascular networks. Such biological processes are subject to precise molecular regulation. So far, the mechanistic insights into understanding how blood vessels grow to the correct position are limited.
View Article and Find Full Text PDFA cost-effective oligo-based barcode system for chromosome identification in longan and lychee.
Hortic Res
January 2025
Key Laboratory of Tropical Fruit Biology (Ministry of Agriculture), South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences, Mazhang District, Zhanjiang 524091, China.
Oligonucleotide (Oligo)-based fluorescence hybridization (FISH) represents a highly effective methodology for identifying plant chromosomes. Longan is a commercially significant fruit species, yet lacking basic chromosomal markers has hindered its cytogenetic research. In this study, we developed a cost-effective oligo-based system for distinguishing chromosomes of longan ( Lour.
View Article and Find Full Text PDFRNAscope Multiplex FISH Signal Assessment in FFPE and Fresh Frozen Tissues: The Effect of Archival Duration on RNA Expression.
J Histochem Cytochem
January 2025
Program in Translational Medicine, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.
Formalin-fixed paraffin-embedded tissue (FFPET), which is the most widely used pathology archive, usually has low-quality DNA and RNA due to extensive nucleic acid crosslinking. RNA fluorescence in situ hybridization (RNA-FISH) has been increasingly utilized in research and clinical settings to diagnose disease pathology. In this study, the effect of RNA degradation over archival time on RNA-FISH signals in FFPET and fresh frozen tissue (FFT) was systematically assessed.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!