Endothelium-dependent production and liberation of kynurenic acid by rat aortic rings exposed to L-kynurenine.

Rat aortic slices produced and liberated the endogenous antagonist of glutamate receptors, kynurenic acid, upon exposure to L-kynurenine. Endothelium-denuded slices did not synthesize any measurable amount of kynurenic acid, indicating its endothelial origin. Aortic kynurenic acid production was diminished by modification of the ionic milieu, hypoxia and hypoglycemia, as well as by L-glutamate and L-aspartate, endogenous glutamate receptor agonists, and aminooxyacetic acid, a non-selective inhibitor of aminotransferases and mitochondrial respiration. These data pave the way for future research aimed to clarify the role of kynurenic acid in the physiology and pathology of the endothelium and vasculature.