We studied the presence of Borrelia burgdorferi sensu lato in Ixodes ricinus ticks collected in woodland areas in the vicinity of Weilburg (Hesse, Germany). Ticks (n = 13,347) were collected in five localities in 1996 and were fixed in 70% ethanol. Out of them, 4404 specimens were analysed individually (n = 406) and in 796 pools of 2-10 specimens (n = 3998) by the polymerase chain reaction (PCR). The calculated minimal infection rate of individual and pooled ticks was 0% in larvae (n = 280), 6.9% in nymphs (n = 3600), 13.9% in males (n = 280) and 20.9% in females (n = 244). Prevalence of B. burgdorferi s. l. in I. ricinus was determined with respect to the abundance and seasonal activity of the ticks.

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