The enzymatic carboxylation of phosphoenolpyruvate by cell-free extracts of Neisseria gonorrhoeae was examined and determined to be similar to the reaction catalyzed by phosphoenolpyruvate carboxylase (PEPC). This was shown by the irreversibility of the reaction and nucleotide independency. The enzyme was found to have some characteristics different from the other bacterial PEPCs reported. The enzyme showed catalytic activity in the presence of cobalt ions as well as magnesium and manganese ions, was not inhibited by succinate in fresh extracts, and displayed a low Michaelis constant for bicarbonate (0.27 mM), as compared with other PEPCs. The significance of this low Michaelis constant is discussed with respect to the growth of the organism and the importance of this enzyme to protein and nucleic acid synthesis.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC234916 | PMC |
| http://dx.doi.org/10.1128/jb.129.1.202-206.1977 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Chloroplast redox state mediates the short-term regulation of leaf isoprene emission.
Tree Physiol
November 2024
Institute of Ecology, College of Urban and Environmental Sciences and Key Laboratory for Earth Surface Processes of Ministry of Education, Peking University, Beijing, 100871, China.
Article Synopsis
- * The study investigates how chloroplast redox state impacts isoprene biosynthesis and examines previous hypotheses about its regulation amidst conflicting evidence regarding cytosolic processes.
- * Experimental results reveal that isoprene emission rates respond consistently to changes in chloroplast redox state across various environmental factors, supporting the idea that this redox state is key in regulating isoprene release.
Metabolomics, phytohormone and transcriptomics strategies to reveal the mechanism of barley heading date regulation to responds different photoperiod.
BMC Genomics
September 2024
State Key Laboratory of Hulless Barley and Yak Germplasm Resources and Genetic Improvement, Lhasa, 850000, China.
Background: The correlation between heading date and flowering time significantly regulates grain filling and seed formation in barley and other crops, ultimately determining crop productivity. In this study, the transcriptome, hormone content detection, and metabolome analysis were performed systematically to analyze the regulatory mechanism of heading time in highland barley under different light conditions. The heading date of D18 (winter highland barley variety, Dongqing18) was later than that of K13 (vernal highland barley variety) under normal growth conditions or long-day (LD) treatment, while this situation will reverse with short-day (SD) treatment.
View Article and Find Full Text PDFSilencing of mitigates the proliferation and migration of vascular smooth muscle cells and vascular intimal hyperplasia by suppressing STAT3/DRP1-mediated mitochondrial fission.
Acta Biochim Biophys Sin (Shanghai)
September 2024
Department of Cardiology, the General Hospital of Western Theater Command, Chengdu 610083, China.
The pathological proliferation and migration of vascular smooth muscle cells (VSMCs) are key processes during vascular neointimal hyperplasia (NIH) and restenosis. Phosphoenolpyruvate carboxy kinase 1 (PCK1) is closely related to a variety of malignant proliferative diseases. However, the role of PCK1 in VSMCs has rarely been investigated.
View Article and Find Full Text PDFImpacts of phosphoenolpyruvate carboxylase gene silencing on photosynthetic efficiency and carbon fixation in Skeletonema costatum.
Gene
January 2025
Key Laboratory of Marine Environment and Ecology, Ministry of Education, College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China; Laboratory for Marine Ecology and Environmental Science, Qingdao Marine Science and Technology Center, Qingdao 266237, China; Frontiers Science Center for Deep Ocean Multispheres and Earth System, Ocean University of China, Qingdao 266100, China.
Diatoms play a crucial role in marine primary productivity through carbon fixation, which is essential for understanding the operation of marine biological pumps and carbon sinks. This study focuses on the phosphoenolpyruvate carboxylase (PEPC) gene, a key enzyme in the carbon assimilation pathway of diatoms, by investigating the consequences of its silencing in Skeletonema costatum. Through this approach, we aimed to clarify the distinct contributions of PEPC to the overall carbon fixation process.
View Article and Find Full Text PDFCarboxymethylation of paramylon derived from Euglena gracilis and its hypoglycemic mechanism in diabetic mice.
Int J Biol Macromol
October 2024
State Key Laboratory of Marine Food Processing and Safety Control, Dalian Polytechnic University, Dalian 116034, Liaoning, China; Academy of Food Interdisciplinary Science, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, Liaoning, China; National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, Liaoning, China; Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian 116034, Liaoning, China; Dalian Key Laboratory for Precision Nutrition, Dalian Polytechnic University, Dalian 116034, Liaoning, China. Electronic address:
Paramylon is a polysaccharide primarily composed of β-1,3-glucan, characterized by its high crystallinity and insolubility in water. Enhancing its water solubility through structural modifications presents an effective strategy to unlock its biological activity. In this study, carboxymethylation was employed to produce carboxymethylated paramylon (CEP) with varying carboxyl concentrations.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!