AI Article Synopsis
- A cDNA of the rice ragged stunt oryzavirus (RRSV) was created to express its protein PS9 in E. coli, resulting in a purified 38kD polypeptide after cleavage.
- Using antiserum raised against the fusion protein, gold immuno-labelling experiments indicated that this 38kD polypeptide is a key protein in the virus spikes.
- Virus transmission tests with brown planthoppers showed that the presence of PS9 inhibited virus transmission, suggesting that spike proteins are crucial for viral infection.
Article Abstract
A cDNA of rice ragged stunt oryzavirus(RRSV) coding for its protein PS9 was prepared and expressed in E.coli as a fusion protein then purified and cleaved by factor-Xa to a 38kD polypeptide. Using the IgG of the antiserum raised against the expressed fusion protein the gold immuno-labelling experiments provided a direct evidence that the 38kD polypeptide is one of the major proteins forming the virus spikes. Virus transmission experiments in brown planthopper fed with PS9 showed the inhibition of transmission of virus by the PS9 protein suggesting that the spike proteins of the virus may be essential for the virus infection.
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