Complete coding sequences of beta-arrestin1 (1A and 1B) were cloned through application of bioinformatics analysis to the dbEST database. beta-arrestin1A was overexpressed in E.coli with partial expression products as inclusion body. Anti-beta-arrestin1 antibodies were prepared by using purified inclusion body. Results also demonstrate that activation of inhibitory G protein mediated by delta and kappa pioid receptors was strongly attenuated by overexpression of beta-arrestin1A in co-transfected 293 cells.
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