AI Article Synopsis
- The cmk2 gene in Schizosaccharomyces pombe produces a 504 amino acid protein kinase related to the calmodulin-dependent protein kinase family.
- Overexpression of the cmk2(+) gene isn't necessary for cell survival but results in blocking the cell cycle at the G2 phase, influencing cdc2 activity.
- Cmk2 protein peaks during the G1/S boundary and helps manage DNA replication issues, allowing extra time to fix replication errors without affecting DNA damage checks.
Article Abstract
The cmk2 gene of Schizosaccharomyces pombe encodes a 504 amino acid protein kinase with sequence homology with the calmodulin-dependent protein kinase family. The cmk2(+) gene is not essential for cell viability but overexpression of cmk2(+) blocks the cell cycle at G2 phase and this inhibition is cdc2-dependent. The Cmk2 is a cytoplasmic protein expressed in a cell cycle-dependent manner, peaking at the G1/S boundary. Overexpression of Cmk2 suppresses fission yeast DNA replication checkpoint defects but not DNA damage checkpoint defects, suggesting that the G2 cell cycle arrest mediated by high levels of Cmk2 provides sufficient time to correct DNA replication alterations.
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| http://dx.doi.org/10.1016/s0014-5793(02)03006-5 | DOI Listing |
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