Introduction: Interesting preliminary data on changes in the neurotrophin system in various digestive diseases have recently begun to emerge.
Aims: To measure changes in messenger RNA (mRNA) levels of neurotrophins and to identify cell types expressing neurotrophins in the pancreas of rats with L-arginine-induced pancreatitis.
Methodology: Rats were killed at time points from 2 hours to 4 weeks after the induction of pancreatitis, and responses were measured by assay.
Results: By RNase protection assay, ciliary neurotrophic factor (CNTF) mRNA expression showed a rapid response (sixfold increase over control) in the inflamed pancreas at 2 hours. The levels of mRNA expression of brain-derived neurotrophic factor (BDNF), glial cell line-derived neurotrophic factor (GDNF), neurotrophin-3 (NT-3), and neurotrophin-4 (NT-4) in the inflamed pancreas reached a peak at 1 week (2.5-fold, twofold, fourfold, and fivefold increase, respectively). By immunohistochemistry, immunoreactivity for all neurotrophins examined was observed in the islets of Langerhans in the control pancreas at all time points, but it was markedly reduced in the islets in the inflamed pancreas at 2 and 6 hours. Acinar and ductal cells, inflammatory cells, and neural elements were immunoreactive for those neurotrophins in the inflamed pancreas from 2 hours to 2 weeks.
Conclusion: The temporal and spatial expression of neurotrophins in the course of experimental pancreatitis suggests that their upregulation is a critical component of the response of the pancreas to injury in this model.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1097/00006676-200207000-00009 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Role of the Pancreatic Islet Microvasculature in Health and Disease.
J Histochem Cytochem
December 2024
Seattle Institute for Biomedical and Clinical Research, and Research Service, Department of Veterans Affairs Puget Sound Health Care System, Seattle, Washington.
The pancreatic islet vasculature comprises microvascular endothelial cells surrounded by mural cells (pericytes). Both cell types support the islet by providing (1) a conduit for delivery and exchange of nutrients and hormones; (2) paracrine signals and extracellular matrix (ECM) components that support islet development, architecture, and endocrine function; and (3) a barrier against inflammation and immune cell infiltration. In type 2 diabetes, the islet vasculature becomes inflamed, showing loss of endothelial cells, detachment, and/or trans-differentiation of pericytes, vessel dilation, and excessive ECM deposition.
View Article and Find Full Text PDFEctopic adrenocortical adenoma in the abdominal wall linked to the head of the pancreas: A case report.
Int J Surg Case Rep
December 2024
Faculty of Medicine, Tishreen University, Latakia, Syria.
Introduction: Ectopic adrenocortical adenomas are infrequent adrenal tumors that deviate from their usual location. Herein, we report a case of an ectopic black adrenocortical adenoma situated in the abdominal wall, intricately linked to the head of the pancreas.
Case Presentation: A 27-year-old female presented to the emergency room with severe right iliac pain, nausea, vomiting, and high fever.
An IL-2 mutein increases regulatory T cell suppression of dendritic cells via IL-10 and CTLA-4 to promote T cell anergy.
Cell Rep
November 2024
Center for Fundamental Immunology, Benaroya Research Institute, Seattle, WA 98126, USA; Department of Immunology, University of Washington School of Medicine, Seattle, WA 981098, USA. Electronic address:
Interleukin-2 (IL-2) variants with increased CD25 dependence that selectively expand Foxp3 regulatory T (T) cells are in clinical trials for treating inflammatory diseases. Using an Fc-fused IL-2 mutein (Fc.IL-2 mutein) we developed that prevents diabetes in non-obese diabetic (NOD) mice, we show that Fc.
View Article and Find Full Text PDFWhole Exome Sequencing Identifies Epithelial and Immune Dysfunction-Related Biomarkers in Food Protein-Induced Enterocolitis Syndrome.
Clin Exp Allergy
November 2024
Genetics, Vaccines and Infections Research Group (GenViP), Instituto de Investigación Sanitaria de Santiago, Universidade de Santiago de Compostela, Santiago de Compostela, Galicia, Spain.
Article Synopsis
- Food protein-induced enterocolitis syndrome (FPIES) is a food allergy mostly affecting infants, characterized by severe vomiting and shock, and its diagnosis can be delayed due to unclear genetic markers.
- This study analyzed the genomes of 41 FPIES patients, identifying significant genetic variants linked to the syndrome, including genes that may influence immune response and gut health.
- The results enhance understanding of FPIES' genetic basis and could pave the way for better diagnosis, treatment, and prevention strategies in the future.
Revisiting the Immunometabolic Basis for the Metabolic Syndrome from an Immunonutritional View.
Biomedicines
August 2024
Madrid Institute for Advanced Studies in Food (IMDEA Food), Carretera Cantoblanco 8, 28049 Madrid, Spain.
Metabolic syndrome (MetS) implies different conditions where insulin resistance constitutes a major hallmark of the disease. The disease incurs a high risk for the development of cardiovascular complications, and takes its toll in regard to the gut-liver axis (pancreas, primary liver and colorectal)-associated immunity. The modulation of immunometabolic responses by immunonutritional factors (IFs) has emerged as a key determinant of the gut-liver axis' metabolic and immune health.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!