AI Article Synopsis
- The paper discusses the potential benefits of gene targeting in a variety of mammalian cells and animals for genetic manipulation.
- It evaluates both empirical data and theoretical concepts related to how transgenes integrate into genomes.
- The conclusion suggests that achieving efficient gene targeting in non-selective systems requires more advancements in understanding homologous recombination.
Article Abstract
The genetic manipulation of mammalian cells and animals would be greatly expedited if gene targeting could be reliably achieved in the widest possible range of host cell types. This paper considers empirical evidence and theoretical considerations associated with transgene integration, and concludes that utilisation of gene targeting in non-selective systems awaits further progress in modelling homologous recombination.
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| http://dx.doi.org/10.1051/rnd:2001102 | DOI Listing |
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