AI Article Synopsis
- The study analyzed lymphocyte subsets in 20 packed red blood cell units before and after using the Pall Leukotrap RC inline filter system, dividing samples into two groups based on preparation methods.
- Group A (low spin/PRP removal) had significantly higher median white blood cell (WBC) counts compared to Group B (high spin/plasma and buffy-coat removal), with p < 0.0001 indicating a strong statistical difference.
- After filtration, lymphocyte counts, especially CD3+, CD3+CD4+, and CD3+CD8+ subsets, were reduced significantly, with all CD19+ lymphocytes dropping below the limit of detection in all units.
Article Abstract
Lymphocyte subsets were determined in 20 packed red blood cell units (PRC) before and after filtration (FPRC) with the Pall Leukotrap RC inline filter system; 10 units were prepared by low spin and platelet rich plasma (PRP) removal (Group A) and 10 with high spin, plasma and buffy-coat (BC) removal (Group B). Flow cytometry was employed for white blood cell (WBC) enumeration and phenotype analysis. Median WBCs in prefiltered units was 2.08 x 10(9) (Group A) vs. 0.8 x 10(9) (Group B) (p < 0.0001). Five Group A and three Group B filtered units had WBC counts above the limit of detection (LD), median values being 25.59 and 3.08 x 10(3), respectively. Whereas CD3+, CD3+CD4+ and CD3+CD8+ lymphocyte subsets were assessable in 20-40% of Group A units, inline filtration of Group B units lowered lymphocytes below the LD of the present study. Post-filtration CD19+ lymphocytes were below the LD in all the 20 units.
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| http://dx.doi.org/10.1016/s1473-0502(02)00009-5 | DOI Listing |
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