Hyaluronan (HA) was depolymerized by partial digestion with testicular hyaluronidase and separated into size-uniform HA oligosaccharides from 4-mers to 52-mers by anion exchange chromatography after removal of the hyaluronidase. The purity and size of each HA oligosaccharide was confirmed by using HPLC analyses, FACE, and ESI-MS. (1)H and (13)C NMR assignments and elemental analyses were obtained for each HA oligosaccharide. Endotoxins, proteins, and DNA were absent or in trace amounts in these HA oligosaccharides. Gram/mg-scale hyaluronan oligosaccharides were obtained from 200 g of HA starting material. These pure, size-uniform, and large range of HA oligosaccharides will be available for investigating important biological functions of HA, such as for the determination of the size(s) of HA oligosaccharides that induce angiogenesis or mediate inflammatory responses, and to interact with HA-binding proteins and receptors both in in vitro and in vivo studies.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1093/glycob/cwf048 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
De novo synthesis of hyaluronic acid with tailored molecular weights using a new hyaluronidase SthHL.
Int J Biol Macromol
December 2024
College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, PR China; State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing Tech University, Nanjing 211816, PR China. Electronic address:
Hyaluronic acid (HA) exhibits various biological activities and functions, mainly governed by its molecular weight (M). Traditional HA degradation methods encounter challenges such as environmental pollution and high costs. Thus, developing a safe cell factory with an efficient regulation strategy for one-step production of specific M HA has attracted significant research interest.
View Article and Find Full Text PDFChemoenzymatic synthesis with the hyaluronan synthase; production of a multitude of defined authentic, derivatized, and analog polymers.
Proteoglycan Res
October 2024
Department of Biochemistry and Physiology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA.
Hyaluronan (HA; [-3-GlcNAc-1-beta-4-GlcA-1-beta] ), an essential matrix polysaccharide of vertebrates and the molecular camouflage coating in certain pathogens, is polymerized by "HA synthase" (HAS) enzymes. Three HAS classes have been identified with biotechnological utility, but only the Class II PmHAS from Type A has been useful for preparation of very defined HA polymers in vitro. Two general chemoenzymatic strategies with different size products are possible: (1) repetitive step-wise extension reactions by sequential addition of a single monosaccharide from a donor UDP-sugar onto an acceptor (or "primer") comprised of a short glycosaminoglycan chain (e.
View Article and Find Full Text PDFPhotoelectrocatalytic degradation of hyaluronic acid and regulation effects of its degradation products on gut microbiota in vitro.
Int J Biol Macromol
December 2024
SKL of Marine Food Processing & Safety Control, National Engineering Research Center of Seafood, Collaborative Innovation Center of Seafood Deep Processing, National & Local Joint Engineering Laboratory for Marine Bioactive Polysaccharide Development and Application, Liaoning Key Laboratory of Food Nutrition and Health, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China. Electronic address:
Hyaluronic acid (HA) has multiple biological activities which are closely related to its molecular weight. In the present study, the photoelectrocatalytic method was established for HA degradation and the influences of bias potentials, HO additions and reaction times on the degradation results were investigated to optimize the reaction condition. Moreover, a series of analysis methods, such as FT-IR and NMR were used to analyze chemical compositions of the degradation products, revealing that photoelectrocatalytic degradation did not damage the structural blocks of HA obviously.
View Article and Find Full Text PDFMass Spectrometry Imaging with Trapped Ion Mobility Spectrometry Enables Spatially Resolved Chondroitin, Dermatan, and Hyaluronan Glycosaminoglycan Oligosaccharide Analysis .
Anal Chem
November 2024
The Rosalind Franklin Institute, Harwell Campus, Didcot OX11 0FA, U.K.
Article Synopsis
- A new mass spectrometry imaging technique allows for the detection and analysis of glycosaminoglycans (GAGs) in retinal tissue, overcoming previous limitations in spatial resolution.
- By using chondroitinase enzymes, researchers can extract and analyze various GAG oligosaccharides, ranging from disaccharides to hexasaccharides, directly from tissue sections.
- The study showcases the ability to resolve isomeric GAG oligosaccharides and their sulfation states across different tissue regions using a method called trapped ion mobility spectrometry (TIMS).
Assessing the therapeutic role of trehalose and hyaluronic acid: implications for patient care.
Int Ophthalmol
October 2024
Department of Ophthalmology, Westchester Medical Center, New York Medical College, Valhalla, NY, USA.
Article Synopsis
- - The systematic review evaluates how trehalose and hyaluronic acid eye drops affect recovery after cataract surgery, specifically looking at tear film stability, ocular surface health, and patient comfort.
- - It includes four studies that found a significant improvement in eye symptoms for patients using a combination of 3% trehalose and 0.15% hyaluronic acid compared to control solutions, measured by various tests like tear break-up time and patient-reported discomfort.
- - Results showed that patients using the treatment reported less discomfort and had better tear film stability and visual acuity improvements post-surgery, suggesting that these drops may enhance recovery following cataract procedures.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!