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Effects of extenders and cryoprotectants on cryopreservation of Thai red junglefowl (Gallus gallus gallus) spermatozoa.
Cryobiology
June 2022
Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen, 40002, Thailand. Electronic address:
Semen cryopreservation is crucial maintain genetic diversity of avian species. Little is known about suitable extenders for post-thaw survival of spermatozoa from Thai red junglefowl (Gallus gallus gallus). Therefore, the present study aimed to compare the suitability of the modified Thai red junglefowl extender (TRJFE) for cryopreservation of Thai red junglefowl spermatozoa with other extenders, including the Schramm extender, the red junglefowl extender (RFE), and the HS1 extender, in terms of sperm viability, motility, and fertility.
View Article and Find Full Text PDFFrom sperm to embryos; lessons learnt from Tim Rowson's career.
Theriogenology
September 2021
Agrotecnio Centre, University of Lleida, 25198, Lleida, Spain; Transfer in Bovine Reproduction SLu, 22300, Barbastro, Spain. Electronic address:
L.E.A.
View Article and Find Full Text PDFAdvances in bovine theriogenology in New Zealand. 2. Breeding management and technologies for improved reproduction.
N Z Vet J
September 2005
University of Melbourne, Department of Veterinary Science, 250 Princes Highway, Werribee 3030, Victoria, Australia.
The importance of submission rates (SR) on conception patterns in dairy herds during a short artificial breeding (AB) programme was first reported in 1973. Subsequent research has focussed on achieving 3-week SRs of 90% through improved detection of oestrus utilising tailpainting and vasectomised bulls fitted with chin-ball harnesses. Despite nutritional limitations of spring pasture as a sole diet, conception rates to first insemination of 65% have been recorded in cycling cows in many trials.
View Article and Find Full Text PDFRelationship between non-return rate and chromatin condensation of deep frozen bull spermatozoa.
Theriogenology
July 2005
Departamento de Reproducción Animal, INIA, Ctra dela Coruña Km 5,9, Madrid 28040, Spain.
This study analyzes the relationship between chromatin condensation and field fertility, expressed as 90 days non-return rate (NRR), of bulls actively used by AI studs. Frozen-thawed semen from five bulls (six ejaculates per bull, three straws per ejaculate), that showed a non-return rate between 60 and 80%, were analyzed to assess sperm chromatin condensation and stability. The chromatin condensation was determined by flow cytometry using propidium iodide as fluorochrome, and the chromatin stability was evaluated by inducing its decondensation with SDS and EDTA.
View Article and Find Full Text PDFDeep freezing of concentrated boar semen for intra-uterine insemination: effects on sperm viability.
Theriogenology
March 2005
Department of Obstetrics and Gynaecology, Faculty of Veterinary Medicine and Animal Science, Swedish University of Agricultural Sciences (SLU), Ullsvägen 14 C, Clinical Centre, P.O. Box 7039 Ultuna, SE-75007 Uppsala, Sweden.
The use of deep-frozen boar semen for artificial insemination (AI) is constrained by the need for high sperm numbers per dose, yielding few doses per ejaculate. With the advancement of new, intra-uterine insemination strategies, there is an opportunity for freezing small volumes containing high sperm numbers, provided the spermatozoa properly sustain cryopreservation. The present study aimed to concentrate (2 x 10(9) spz/mL) and freeze boar spermatozoa packed in a 0.
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