Introduction of lanthanide(III) chelates to oligopeptides on solid phase.

Bioconjug Chem

PerkinElmer Life Sciences, Wallac Oy, P.O. Box 10, FIN-20101 Turku, Finland.

Published: August 2003

The synthesis of oligopeptide building blocks for the introduction of nonluminescent and luminescent lanthanide(III) chelates to the oligopeptide structure on the solid phase is described. The oligopeptide conjugates synthesized were used in DELFIA-based receptor binding assay (motilin) as well as in LANCE time-resolved fluorescence quenching assay (caspase-3).

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http://dx.doi.org/10.1021/bc025521lDOI Listing

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