In order to clarify the specific identity of the etiological agent of human gnathostomosis in Nayarit State, Mexico, morphological and molecular studies were conducted on advanced third stage larvae obtained from human and fish tissue. Cathorops fuerthii from Agua Brava lagoons complex, was the only fish species found to be infected among four species surveyed. Morphological variability does not allow specific identification of the larvae. Internal transcribed spacer 2 of the ribosomal DNA was sequenced for six larvae (five from fish, one from human tissue). Low divergence in the sequences of Nayarit larvae and Gnathostoma binucleatum (0.24% or less) indicate that the larvae examined belong to this species.
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http://dx.doi.org/10.1016/s1383-5769(02)00014-4 | DOI Listing |
Vet Parasitol
August 2014
Departamento de Ciencias Biológicas, Facultad de Estudios de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, Mexico. Electronic address:
This study aims to describe some of the unknown pathological and parasitological traits of experimental feline gnathostomosis. Thirteen female cats were orally inoculated with 30 advanced third-stage Gnathostoma binucleatum larvae and were euthanized at various post-infection (p.i.
View Article and Find Full Text PDFAm J Trop Med Hyg
June 2012
Universidad Autónoma de Nayarit, Unidad Académica de Ciencias Químico, Biológicas y Farmacéuticas; Tepic Nayarit, Mexico.
Gnathostomiasis is an emerging systemic parasitic disease acquired by consuming raw or uncooked fresh-water fish infected with the advanced third-stage larvae of Gnathostoma spp. This disease is endemic to the Pacific region of Mexico, and one of its etiologic agents has been identified as Gnathostoma binucleatum. We characterized the humoral immune response of patients clinically diagnosed with gnathostomiasis by detecting total IgM, IgE, and IgG class and subclasses against a crude extract of the parasite by Western blotting.
View Article and Find Full Text PDFJ Food Prot
May 2011
Laboratorio de Parasitología, Secretaria de Investigación y Posgrado, Universidad Autónoma de Nayarit, Mexico.
To diminish the risk of transmission to humans of advanced third-stage larvae (A3L) of Gnathostoma binucleatum in fish foods, we evaluated the effects of some physical factors on larval viability. A3L protected within fish meatballs were subjected to freezing, refrigeration, boiling, dry heat, and immersion in lemon juice. By freezing, larvae were killed in 48 h, by refrigeration after 30 days, by boiling in 4 min, and by broiling for 60 min.
View Article and Find Full Text PDFExp Parasitol
January 2011
Laboratorio de Parasitología, Secretaría de Investigación y Posgrado, Universidad Autónoma de Nayarit, México.
Lesions and antibody kinetics produced by inoculation of Gnathostoma binucleatum larvae into dogs are described, as well as the morphology of the recovered parasites. In four out of five infected bitches parasite phases were found in the stomach. Only one bitch eliminated eggs and adult parasite phases in feces.
View Article and Find Full Text PDFParasitol Res
December 2007
CEMIC, Universidad Autonoma de Nayarit, Tepic, Nayarit, Mexico.
Human gnathostomosis is a severe public health problem in the State of Nayarit, Mexico. Between 1995 and 2005, the registration of human cases numbered 6,328, which makes it one of the largest focal points of the disease in the country. The present study determined the presence of natural hosts of Gnathostoma binucleatum larvae at the Laguna de Agua Brava in Nayarit, Mexico.
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