A novel and sensitive test for rapid determination of water toxicity.

Environ Toxicol

Department of Food Engineering and Biotechnology, Technion Institute, Haifa 32000, Israel.

Published: September 2002

AI Article Synopsis

  • A new bioassay using the luminescent bacterium Photobacterium leiognathi can detect chemical toxicants in water at very low concentrations, outperforming existing tests like the Microtox assay.
  • The test is capable of identifying a wide range of toxic agents, including heavy metals and pesticides, and can distinguish between different types of contaminants.
  • It operates effectively at typical environmental temperatures and does not require refrigeration, making it convenient and cost-effective for on-site testing.

Article Abstract

The performance of a novel, rapid, and sensitive test for detecting chemical toxicants in water is described in this article. The bioassay utilizes a highly sensitive variant of the luminescent bacterium Photobacterium leiognathi that allows the detection in water at levels below milligrams per liter of diverse groups of toxicants, including heavy metals, pesticides, PCBs, polycyclic aromatic hydrocarbons, and fuel traces. For most toxic agents reported in this study, the new assay was markedly more sensitive than the Microtox(trade mark) Vibrio fischeri assay according to the bacterial bioluminescence toxicity data reported in the literature. Additional features of the new bioassay include the ability to discriminate between cationic heavy metals and organic toxicants and the option of being run at ambient temperatures (18 degrees C-27 degrees C), thereby enabling on-site testing with low-cost luminometers. In addition, the stability of the freeze-dried bacterial reagent preparation at ambient temperatures precludes the need for refrigeration or freezing during shipment, which contributes to further reducing overall operational costs.

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http://dx.doi.org/10.1002/tox.10060DOI Listing

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