AI Article Synopsis
- The study measured glutathione (GSH) and its oxidized form (GSSG) in spruce needles using ion trap and triple stage quadrupole mass spectrometers.
- Stability tests revealed the presence of various forms of GSH, including dimeric and trimeric, along with GSSG and related compounds in aqueous solutions and needle extracts.
- An assay was developed for detecting low levels of GSH in plant tissues, leveraging the sensitivity of liquid chromatography tandem mass spectrometry (LC/MS/MS), with preliminary results presented.
Article Abstract
For the determination of glutathione (GSH) and its oxidized form (GSSG) in spruce needles their electrospray mass and MS/MS spectra were recorded with an ion trap mass spectrometer (ITMS, LCQ, Finnigan) and a triple stage quadrupole mass spectrometer (TSQ, Quattro II, Micromass). A study of the stability of GSH in aqueous solutions shows the presence of dimeric and trimeric forms of GSH, as well as GSSG, GSH-sulfonate and GSH-sulfinic acid. The same components were also found in extracts of spruce needles. We developed an assay which is suitable for monitoring low concentrations of GSH and similar compounds in plant tissues, employing the sensitivity and specificity of LC/MS/MS. Preliminary results on the mass spectrometric determination of GSH in spruce needles are given.
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| http://dx.doi.org/10.1002/rcm.700 | DOI Listing |
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