To identify the roles of pyridoxine-5'-phosphate (PNP) oxidase in epileptogenesis and the recovery mechanisms in spontaneous seizure, a chronological and comparative analysis of PNP oxidase expression was conducted. PNP oxidase immunoreactivity in a preseizure group of seizure-sensitive (SS) gerbils was detected more strongly than that in a seizure-resistant (SR) group. The density of PNP oxidase immunoreactivity in a 30 min postictal group was significantly lower than that in a preseizure group. In a 12 hr postictal group, PNP oxidase immunodensity had recovered to a preseizure level. The overexpression of PNP oxidase in the hippocampus of preseizure SS gerbils suggests that PNP or pyridoxal 5'-phosphate plays an important role in the modulation of glutamic acid decarboxylase activity and gamma-aminobutyric acid reuptake as mediated by membrane transporter via neurons. In addition, this change in the PNP oxidase immunoreactivity following seizure may be a compensatory response designed to reduce epileptic activity in this animal.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/jnr.10280 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Bimetallic CuMn nanozyme-enzyme microsystem for efficient dimethyl phthalate degradation.
Int J Biol Macromol
January 2025
Mianyang 404 Hosp, Med Lab, Mianyang 621000, Sichuan, China.
Article Synopsis
- A new nanozyme-enzyme microsystem (CMAC@Lipase) was developed using copper-manganese aminoclays combined with lipase for enhanced pollutant treatment in water.
- The system showcased laccase-like activity, allowing it to efficiently break down pollutants, such as dimethyl phthalate, while maintaining significant stability over multiple uses.
- This research offers innovative strategies for creating effective solutions for environmental pollution management in aquatic systems.
AIE multifunctional probe empowering colorimetric-fluorescence dual-mode biosensor for early diabetic screening.
Biosens Bioelectron
February 2025
College of Material, Chemistry and Chemical Engineering, Key Laboratory of Organosilicon Chemistry and Material Technology, Ministry of Education, Hangzhou Normal University, Hangzhou, 311121, Zhejiang, China. Electronic address:
Article Synopsis
- Diabetes mellitus (DM) is a chronic condition marked by high blood glucose levels, but traditional biomarkers often fail to detect early stages due to their variability.
- A novel dual-mode biosensor using α-glucosidase and modified copper nanoclusters has been developed for improved early diabetes screening, minimizing influences of blood glucose fluctuations.
- This biosensor significantly increases detection accuracy, reducing false positives by 50% and false negatives by 25%, making it a reliable tool for clinical evaluations of abnormal glucose levels.
[Molecular mechanism of high-altitude hypoxia-induced lipid metabolism disorder in mouse spleen tissue].
Nan Fang Yi Ke Da Xue Xue Bao
October 2024
College of Medicine of Qinghai University, Xining 810016, China.
Objective: To investigate the molecular mechanism of lipid metabolism disorder in mouse spleen tissues due to high-altitude hypoxia.
Methods: Ten C57BL/6 male mice were randomly divided into normoxia group (maintained at an altitude of 400 m) and high-altitude hypoxia group (maintained at 4200 m) for 30 days (=5). Lipidomics and metabolomics analyses of the spleen tissue of the mice were conducted using liquid chromatography-mass spectrometry (LC-MS) to identify the differential metabolites, which were further analyzed by KEGG enrichment and pathway analyses, and the differential genes were screened through transcriptome sequencing.
Interaction of Tri-Cyclic Nucleobase Analogs with Enzymes of Purine Metabolism: Xanthine Oxidase and Purine Nucleoside Phosphorylase.
Int J Mol Sci
September 2024
Division of Biophysics, Institute of Experimental Physics, Faculty of Physics, University of Warsaw, ul. Pasteura 5, PL-02-093 Warsaw, Poland.
Fluorescent markers play important roles in spectroscopic and microscopic research techniques and are broadly used in basic and applied sciences. We have obtained markers with fluorescent properties, two etheno derivatives of 2-aminopurine, as follows: 1,N-etheno-2-aminopurine (1,N-ε2APu, ) and N,3-etheno-2-aminopurine (N,3-ε2APu, ). In the present paper, we investigate their interaction with two key enzymes of purine metabolism, purine nucleoside phosphorylase (PNP), and xanthine oxidase (XO), using diffraction of X-rays on protein crystals, isothermal titration calorimetry, and fluorescence spectroscopy.
View Article and Find Full Text PDFIdentification of YigL as a PLP/PNP phosphatase in .
Appl Environ Microbiol
September 2024
Department of Applied Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University, Furou-chou, Chikusa, Nagoya, Aichi, Japan.
In various organisms, the coenzyme form of vitamin B, pyridoxal phosphate (PLP), is synthesized from pyridoxine phosphate (PNP). Control of PNP levels is crucial for metabolic homeostasis because PNP has the potential to inhibit PLP-dependent enzymes and proteins. Although the only known pathway for PNP metabolism in involves oxidation by PNP oxidase, we detected a strong PNP phosphatase activity in cell lysate.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!