Human B1F was cloned from a human liver cDNA library by yeast one-hybrid screening and characterized. It is a transcription factor which belongs to nuclear receptor superfamily. The cDNA segment 450-930 of human liver transcription factor hB1F was cloned into the pGEX-3X expression vector and was expressed in E.coli. The purified GST-fused hB1F, named GST-CS, were used to immunize mice to get polyclonal antibodies to hB1F. The antiserum showed specificity to hB1F after the purification by GST-Sepharose 4B. It can be used in further investigations of the structure and function of hB1F.
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