AI Article Synopsis
- The study evaluated how different carbon and nitrogen sources affect phytase production by Mitsuokella jalaludinii and aimed to optimize rice bran and soybean milk concentrations in the growth medium.
- Replacing glucose and other common ingredients with rice bran and soybean milk significantly boosted phytase production, with optimal concentrations found to be 15% RB and 20% SM, among other combinations.
- The findings suggest that enhancing phytase production through genetic manipulation could lead to commercial applications, highlighting the importance of this research.
Article Abstract
Aims: The effects of different carbon and nitrogen sources on phytase production by Mitsuokella jalaludinii were evaluated and the optimization of rice bran (RB) and soybean milk (SM) concentrations in the medium for phytase production was also determined.
Methods And Results: Replacement of glucose, cellobiose and starch in MF1 medium by RB or palm kernel cake and replacement of trypticase peptone and yeast extract in the medium by SM or enzymatic digested soybean milk significantly increased the phytase production by M. jalaludinii. The optimal concentrations of RB and SM in the medium for phytase production were 15% RB and 20% SM or 20% RB and 10% SM or 20% RB and 20% SM and the phytase activities in the media were 12.53, 12.93 and 12.75 U g-1 culture broth, respectively.
Conclusions, Significance And Impact Of The Study: The high production of phytase by M. jalaludinii warrants further research to increase its yield by genetic manipulation for commercial application.
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| http://dx.doi.org/10.1046/j.1472-765x.2002.01153.x | DOI Listing |
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