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Detection and phylogenetic analysis of blood-associated pathogens from spleen samples of wild raccoons (Procyon lotor) in Germany.
Sci Rep
December 2024
Department of Zoology, Musée National d'Histoire Naturelle, Luxembourg, Luxembourg.
Raccoons (Procyon lotor) originated in North America and have been introduced to Europe. Due to their close contact with human settlements, they are important reservoirs for zoonotic pathogens, such as Baylisascaris procyonis. The relevance and prevalence of vector-borne pathogens have not yet been fully elucidated.
View Article and Find Full Text PDFApplication of deep learning models on single-cell RNA sequencing analysis uncovers novel markers of double negative T cells.
Sci Rep
December 2024
Department of Biomedical Sciences and Pathobiology, Virginia Tech, Blacksburg, VA, USA.
Double negative T (DNT) cells are a unique subset of CD3 + TCRαβ + T lymphocytes that lack CD4, CD8, or NK1.1 expression and constitute 3-5% of the total T cell population in C57BL/6 mice. They have increasingly gained recognition for their novel roles in the immune system, especially under autoimmune conditions.
View Article and Find Full Text PDFHazardous effects of heavy metal pollution on Nile tilapia in the aquatic ecosystem of the Eastern Delta in Egypt.
BMC Vet Res
December 2024
Department of Zoology, Faculty of Science, Benha University, Benha, 13518, Egypt.
Introduction: Heavy metal pollution threatens the biodiversity and ecological equilibrium of the Nile River. This study investigates the impact of heavy metal pollution on aquatic animals such as Nile tilapia (Oreochromis niloticus) in the Damietta branch of the River Nile and El-Rayah El-Tawfeeky canal in Benha City in Egypt.
Methods: Fish and water samples were collected from the Damietta branch and El-Rayah El-Tawfeeky during the fall of 2022.
A vaccine combining ORF132 and ORF25 expressed by Saccharomyces cerevisiae induces protective immunity in Carassius auratus gibelio against CyHV-2.
Fish Shellfish Immunol
December 2024
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, School of Marine Sciences, Ningbo University, Ningbo 315211, China; Laboratory of Biochemistry and Molecular Biology, School of Marine Sciences, Ningbo University, Ningbo 315211, China; Key Laboratory of Marine Biotechnology of Zhejiang Province, Ningbo University, Ningbo 315211, China. Electronic address:
CyHV-2 is the pathogen of herpesvirus haematopoietic necrosis (HVHN), resulting in significant economic losses in crucian carp. Although multiple oral vaccines have been developed to prevent CyHV-2, they have not achieved ideal protective effects. To improve the protective effect of oral vaccine, a combination vaccine was conducted by mixing recombinant Saccharomyces cerevisiae displaying ORF132 or ORF25 on the cell surface in a 1:1 ratio.
View Article and Find Full Text PDFX-ray irradiation reduces ATP-dependent activation of NLRP3 inflammasome by inhibiting TWIK2 activity in macrophages.
Immunol Lett
December 2024
Department of Emergency, Affiliated Hospital of Zunyi Medical University, 563003 Zunyi, Guizhou, China. Electronic address:
Background: The spleen, as the body's largest peripheral immune organ and a crucial source of circulating monocytes, plays a significant role in the acute inflammatory response of spleen-derived macrophages to diseases. Therefore, studying the impact and mechanism of X-ray irradiation on spleen-derived macrophages' inflammatory responses is of great importance.
Method: Extracted and identified mice splenic macrophages were divided into four groups: control group, LPS and ATP co-stimulated non-irradiated group, LPS and ATP co-stimulated group irradiated after 6h, and LPS and ATP co-stimulated group irradiated after 12h.
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