Studied were biochemically and serologically a total of 10 Mycoplasma cultures isolated from milk of mastitis-affected cows (clinical and subclinical forms). The organisms were differentiated as belonging to Mycoplasmataceae (M. alkalescens species), and Acholeplasmataceae (A. laidlowii species). Demonstrated was also the presence of mixed cultures including M. alkalescens and A. laidlawii. The organisms specified as A. laidlowii showed atypical properties so far as some biochemical indices were concerned.
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