AI Article Synopsis
- The study confirms that PPAR alpha and PPAR delta interact with specific coactivators in intestinal cells, suggesting a pivotal role in gut metabolism.
- The researchers isolated genes for two key coactivators, CREB-binding protein (CBP) and p300, noting that p300 is more widely expressed across tissues.
- Findings indicate that p300 interacts more efficiently with PPAR alpha and PPAR delta compared to CBP when specific ligands are present, highlighting its importance in intestinal function.
Article Abstract
We have previously reported that several genes related to intestinal fatty acid and vitamin A metabolism are coordinately regulated by peroxisome proliferator-activated receptor (PPAR) [Arch. Biochem. Biophys. 389 (2001) 41; Biochim. Biophys. Acta 1531 (2001) 68]. In this study, we demonstrated that PPAR alpha and PPAR delta interacted with endogenous coactivators in intestinal cell line, Caco-2 in a ligand specific manner. We isolated rat cDNA clones encoding the nuclear receptor interaction domains of the two transcriptional coactivators, CREB-binding protein (CBP) and p300. Expression level of CBP mRNA was relatively low in the small intestine, while p300 mRNA was ubiquitously expressed in various tissues including the small intestine in the rat. Southern blot analysis revealed that these coactivators were encoded by different genes. Mammalian two-hybrid assays in Caco-2 cells revealed that p300 interacted with PPAR alpha or PPAR delta in the presence of their specific ligands more efficiently than CBP did. These results suggest that the major intestinal coactivator, p300 strongly interacts with PPAR alpha and PPAR delta.
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| http://dx.doi.org/10.1016/s0378-1119(02)00625-x | DOI Listing |
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