Hypoxia activates a background conductance in freshly isolated pulmonary arterial endothelial cells of the rat.

Utilising the patch-clamp recording technique we have demonstrated for the first time the effects of hypoxia on the background current in pulmonary arterial endothelial cells. Electrophysiological studies revealed the presence of a novel oxygen-sensitive, non-selective cation conductance (I(NSC)) in these cells. The inward component of I(NSC) was significantly potentiated by hypoxia. Both the inward and outward components of I(NSC) were inhibited by both La(3+) and Gd(3+). Hypoxic activation of I(NSC) may provide an important Ca(2+) influx pathway essential for the release of a pulmonary-selective vasoconstrictor pivotal to the sustained phase of hypoxic pulmonary vasoconstriction.