A rapid, precise and time saving high performance liquid chromatographic method has been developed and validated for the simultaneous determination of nicotinamide and 4-aminobenzoic acid in pharmaceutical preparation. The method involves isocratic elution of mobile phase through column in a reverse phase chromatography with UV detection at 254 nm. The ranges of quantification for nicotinamide and 4-aminobenzoic acid were 11-34 microg ml(-1) and 37-113 microg ml(-1), respectively. Investigating specificity, linearity, precision, accuracy, robustness and ruggedness performed the validation of the method.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/s0731-7085(02)00039-0 | DOI Listing |
Publication Analysis
Top Keywords
nicotinamide 4-aminobenzoic
12
4-aminobenzoic acid
12
determination nicotinamide
8
acid pharmaceutical
8
pharmaceutical preparation
8
microg ml-1
8
preparation rapid
4
rapid precise
4
precise time
4
time saving
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!