The mouse Na+/taurocholate cotransporting polypeptide transiently expressed in COS-7 cells caused sodium-dependent uptake of [3H]taurocholic acid with Km and Vmax values of 18 microM and 102 pmol/mg protein/min, respectively. This Km value is comparable to that for rat NTCP and higher than that for human NTCP. Substrate specificity was evaluated by measuring inhibitory effects of unlabeled bile acids on [3H]taurocholic acid transport.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1271/bbb.66.1116 | DOI Listing |
Publication Analysis
Top Keywords
mouse na+/taurocholate
8
na+/taurocholate cotransporting
8
cotransporting polypeptide
8
cos-7 cells
8
[3h]taurocholic acid
8
characterization cloned
4
cloned mouse
4
polypeptide transient
4
transient expression
4
expression cos-7
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!