Aim: To study cytogenetics of peripheral blood lymphocytes in patients with systemic lupus erythematosus (SLE) in the course of therapy with a selective immunodepressant sandimmun and universal cytostatic cyclophosphamide.
Material And Methods: 30 women with lupus-nephritis and nephrotic syndrome received sandimmun in a dose 2.5-7 mg/kg plus 10-20 mg prednisolone for a month with gradual lowering of sandimmun dose to 2.5-5 mg/kg and prednisolone to 0-10 mg/day. 10 patients with lupus-nephritis were given cyclophosphamide in a dose 1000 mg as a single intravenous drip once a month for 6 months in combination with 30-40 mg prednisolone for a month with the dose reduction to 20 mg/day. Sandimmun and cyclophosphamide effects on mutagenesis were studied in 72-120-h PHA-stimulated lymphocyte cultures. Chromosomal aberrations (CA) were assessed according to A.F. Zakharov's classification and proposals of International workshop in Melburn (1993).
Results: Sandimmun therapy on day 14 did not increase CA frequency but led to appearance of "premature chromosome condensation phenomenon" (PCC). PCC diminished after one month of sandimmun therapy and disappeared after 3-6 months. Cyclophosphamide provoked a rise in the incidence of both chromatide aberrations and CA.
Conclusion: Sandimmun does not increase CA incidence in lymphocytes. On the contrary, it promoted the fall in this incidence while syclophosphamide stimulated CA and chromatide aberrations. This trend was observed till the end of cyclophosphamide administration.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
TIGIT and PVRIG are immune checkpoints co-expressed on activated T and NK cells, contributing to tumor immune evasion. Simultaneous blockade of these pathways may enhance therapeutic efficacy, positioning them as promising dual targets for cancer immunotherapy. This study aimed to develop a bispecific antibody (BsAb) to co-target TIGIT and PVRIG.
View Article and Find Full Text PDFBlood leukocyte-based clusters in patients with traumatic brain injury.
Front Immunol
January 2025
Department of Critical Care Medicine, West China Hospital, Sichuan University, Chengdu, Sichuan, China.
Background: Leukocytes play an important role in inflammatory response after a traumatic brain injury (TBI). We designed this study to identify TBI phenotypes by clustering blood levels of various leukocytes.
Methods: TBI patients from the Medical Information Mart for Intensive Care-III (MIMIC-III) database were included.
Activity-Regulated Cytoskeleton-Associated Protein Gene Expression Is Associated With High Infiltration of Stromal Cells and Immune Cells, but With Less Cancer Cell Proliferation and Better Overall Survival in Estrogen Receptor-Positive/Human Epidermal Growth Factor Receptor 2-Negative Breast Cancers.
World J Oncol
February 2025
Breast Surgery, Department of Surgical Oncology, Roswell Park Comprehensive Cancer Center, Buffalo, NY, USA.
Background: Peritumoral lidocaine infiltration prior to excision is associated with better survival in breast cancer (BC), which led us to hypothesize that innervation to the tumor affects its biology and patient survival. Activity-regulated cytoskeleton-associated protein (ARC) gene expression is known to be regulated by neuronal activity. Therefore, we studied the clinical relevance of ARC gene expression as a surrogate of neuronal activity in BC.
View Article and Find Full Text PDFDiscordant Responses Between Imaging Examination and Surgical Pathology of Head and Heck Squamous Cell Carcinoma After Neoadjuvant Immunotherapy Combined With Chemotherapy.
World J Oncol
February 2025
Department of Head and Neck Surgery, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Background: We here investigated the value of imaging examination in evaluating tumor remission-based surgery in patients with head and neck squamous cell carcinoma (HNSCC), who had undergone neoadjuvant immunotherapy combined with chemotherapy (NICC).
Methods: HNSCC patients who underwent NICC and surgery from May 2021 to September 2023 were retrospectively analyzed. All patients had to undergo imaging examination evaluation, including enhanced computed tomography (CT) and enhanced magnetic resonance (MR) imaging before and after NICC.
An exploration of the natural and acquired immunological mechanisms to high-risk human papillomavirus infection and unmasking immune escape in cervical cancer: A concise synopsis.
Tzu Chi Med J
December 2024
Department of Obstetrics and Gynecology, College of Medicine, University of Babylon, Hilla, Iraq.
The most common STD that triggers cervical cancer is the human papillomavirus. More than 20 types of human papillomavirus (HPV) can induce uterine cervical cancer. Almost all women acquire genital HPV infection soon after their first intercourse, with most of them clearing the virus within 3 years.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!