Diabetic rats display exaggerated hyperalgesic behavior in response to noxious stimuli that may model aspects of painful diabetic neuropathy. This study examined the contribution of spinal prostaglandin production to this exaggerated hyperalgesic behavior. Rats were implanted with spinal dialysis probes and received noxious stimulation to the hind paw by subcutaneous injection of 0.5% formalin solution. Prostaglandin E(2) (PGE(2)) was measured in dialysates of lumbar spinal cerebrospinal fluid concurrent with behavioral responses to formalin injection. In separate experiments, formalin-evoked behavioral responses were measured after intrathecal delivery of either a cyclooxygenase inhibitor or an EP(1) receptor antagonist, and cyclooxygenase protein was measured in spinal cord homogenates. Diabetic rats exhibited exaggerated behavioral responses to paw formalin injection and a concurrent prolongation of formalin-evoked PGE(2) release. Formalin-evoked behavioral responses were dose-dependently reduced in diabetic rats by spinal delivery of a cyclooxygenase inhibitor or an EP(1) receptor antagonist. Protein levels of cyclooxygenase-2 were elevated in the spinal cord of diabetic rats, whereas cyclooxygenase-1 protein was reduced. Hyperalgesic behavior in diabetic rats is associated with both increased cyclooxygenase-2 protein and cyclooxygenase-mediated PGE(2) release. Spinal delivery of selective inhibitors of cyclooxygenase-2 or antagonists of prostaglandin receptors may have therapeutic potential for treating painful diabetic neuropathy.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.2337/diabetes.51.7.2249 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Assessing the impact of intermittent fasting and a low-carbohydrate-high-protein diet on metabolic health and pancreatic histopathology in type 2 diabetic rat model.
Int J Health Sci (Qassim)
January 2025
Department of Food Science and Human Nutrition, College of Agriculture and Food, Qassim University, 51452 Buraidah, Saudi Arabia.
Objective: The current study was conducted to investigate the effect of intermittent fasting (IF) with a low-carbohydrate-high-protein (LCHP) diet on blood glucose control in streptozotocin (STZ)-nicotinamide-induced type 2 diabetic rats (DR).
Methods: Thirty male Wistar rats were divided into six groups ( = 5) including a group of normal rats (NR) that received a control diet (CD) (50% carbohydrates, 17% protein, and 33% fat) with (AL) feeding. The remaining 5 groups were DR injected with STZ and fed on CD or LCHP diet (40% carbohydrates, 30% protein, and 30% fat) for 6 weeks, either AL or IF (with a time-restricted feeding of 16 h followed by 8 h feeding period).
Comparison of the efficacy of curcumin and its nano formulation on dexamethasone-induced hepatic steatosis, dyslipidemia, and hyperglycemia in Wistar rats.
Heliyon
December 2024
Department of Clinical Pharmacology, Faculty of Medicine, Sohag University, Sohag, Egypt.
Background And Objective: Insulin resistance is a primary feature of type 2 diabetes. This study compared the effects of curcumin and its nanoformulation on insulin resistance, fasting blood sugar, liver function, GLUT4, lipid profile, and oxidative stress in the liver and pancreas in a diabetic model.
Methods: Thirty male Wistar rats were divided into five groups: a control group, a diabetic group, a diabetic group treated with metformin (40 mg/kg), a diabetic group treated with curcumin (100 mg/kg), and a diabetic group treated with curcumin NPs (100 mg/kg).
Enhancing diabetic rat peripheral nerve conduction velocity parameters through the potent effects of MitoTEMPO.
Heliyon
December 2024
Baskent University, Faculty of Medicine, Department of Biophysics, Ankara, Türkiye.
The efficacy of MitoTEMPO, a mitochondria-targeted antioxidant, in altering nerve fiber conduction properties within the sciatic nerve of streptozotocin (STZ)-induced diabetic rats, a model for diabetic neuropathy characterized by myelinated fiber atrophy and nodal abnormalities. Utilizing the STZ-induced diabetic rat model, we assessed the impact of MitoTEMPO on nerve function through compound action potential (CAP) analysis and histological evaluation. Key indicators such as maximum depolarization (MD), CAP area, and conduction velocity distribution (CVD) were measured to gauge MitoTEMPO's neuroprotective effects, alongside physical parameters like weight and blood glucose levels.
View Article and Find Full Text PDFThe cumulative effect of ellagic acid and carnosic acid attenuates oxidative events during diabetic wound healing: in different applications and on different days.
Turk J Biol
October 2024
Department of Biology, Faculty of Science, Gazi University, Ankara, Turkiye.
Background/aim: The hyperglycemic environment in diabetes disrupts normal wound-healing processes, leading to chronic wounds. This study investigated whether the combination of the phenolic compounds ellagic acid and carnosic acid shows synergistic effects on diabetic wound healing and oxidative parameters in diabetic rats.
Materials And Methods: Diabetic rats were divided into control, untreated, Carbopol 974P treated, topical treatment, and oral gavage treatment groups.
In Situ Formation of Hydrogels Loaded with ZnO Nanoparticles Promotes Healing of Diabetic Wounds in Rats.
ACS Omega
December 2024
The Third Affiliated Hospital of Anhui Medical University, The First People's Hospital of Hefei, Anhui, Hefei 230000, China.
The challenge of healing diabetic skin wounds presents a significant hurdle in clinical practice and scientific research. In response to this pressing concern, we have developed a temperature-sensitive, in situ-forming hydrogel comprising poly(-isopropylacrylamide---butyl acrylate) -poly(ethylene glycol) -poly(-isopropylacrylamide--butyl acrylate) copolymer, denoted as PEP, in combination with zinc oxide nanoparticles, forming what we refer to as PEP-ZnO hydrogel. The antimicrobial properties of the PEP-ZnO hydrogel against methicillin-resistant were rigorously assessed by using the bacteriostatic banding method.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!