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Evaluation of chlorine dioxide exposure in an Australian gnotobiotic mouse research facility.
J Occup Environ Hyg
October 2024
School of the Environment, Faculty of Science, The University of Queensland, Brisbane, Queensland, Australia.
Exposure to chlorine dioxide by staff working in a gnotobiotic mouse facility at an Australian research institute was measured to determine whether current controls were sufficient to ensure their exposure remains below the current Australian workplace exposure standard. A combination of workplace surveys, interviews with workers, and personal sampling was undertaken to understand the workplace, identify higher-risk tasks, and measure the concentration of chlorine dioxide in the air where the workers conduct routine tasks involving the use of a chlorine dioxide-based disinfectant. Personal sampling utilized the validated Occupational Safety and Health Administration (OSHA) method ID-202, with minor alterations.
View Article and Find Full Text PDFSterility testing of germ-free mouse colonies.
Front Immunol
December 2023
Center for Thrombosis and Hemostasis (CTH), University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany.
In biomedical research, germ-free and gnotobiotic mouse models enable the mechanistic investigation of microbiota-host interactions and their role on (patho)physiology. Throughout any gnotobiotic experiment, standardized and periodic microbiological testing of defined gnotobiotic housing conditions is a key requirement. Here, we review basic principles of germ-free isolator technology, the suitability of various sterilization methods, and the use of sterility testing methods to monitor germ-free mouse colonies.
View Article and Find Full Text PDFEquipment and Methods for Concurrently Housing Germfree and Gnotobiotic Mice in the Same Room.
J Am Assoc Lab Anim Sci
September 2023
Comparative Medicine, Research, and Innovation, University of South Florida.
Here, we combined the use of 2 technologies that have not previously been used together-a positively pressurized isolator IVC (IsoIVC-P) and a modular isolator with integrated vaporized hydrogen peroxide (VHP) technology???to develop highly tractable and scalable methods to support long-term maintenance of germfree mouse colonies and the concurrent use of germfree and gnotobiotic mice in the same room. This space-efficient system increases the practicality of microbiome studies. Specifically, the exterior surfaces of microbially similar IsoIVC-P were sterilized by using VHP prior to opening the cages and handling the mice therein.
View Article and Find Full Text PDFMicrobiome complexity shapes metabolism.
PLoS Biol
September 2022
Microbiology Department, Institute for Immunology, and Institute for Diabetes, Obesity, and Metabolism, Perelman School of Medicine, University of Pennsylvania, Philadelphia, United States of America.
Equipped with a novel isolator-housed metabolic cage system, a study in PLOS Biology assessed how the metabolism of mice harboring a defined minimal microbial community (OligoMM12) differs from that of germ-free and conventionally colonized mice.
View Article and Find Full Text PDFMetabolic reconstitution of germ-free mice by a gnotobiotic microbiota varies over the circadian cycle.
PLoS Biol
September 2022
Laboratory for Mucosal Immunology, Institute of Food, Nutrition and Health, Department of Health Sciences and Technology, ETH Zürich, Zürich, Switzerland.
The capacity of the intestinal microbiota to degrade otherwise indigestible diet components is known to greatly improve the recovery of energy from food. This has led to the hypothesis that increased digestive efficiency may underlie the contribution of the microbiota to obesity. OligoMM12-colonized gnotobiotic mice have a consistently higher fat mass than germ-free (GF) or fully colonized counterparts.
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