The presence of ingredients derived from genetically modified organisms (GMOs) in food products in the market place is subject to a number of European regulations that stipulate which product consisting of or containing GMO-derived ingredients should be labeled as such. In order to maintain these labeling requirements, a variety of different GMO detection methods have been developed to screen for either the presence of DNA or protein derived from (approved) GM varieties. Recent incidents where unapproved GM varieties entered the European market show that more powerful GMO detection and identification methods will be needed to maintain European labeling requirements in an adequate, efficient, and cost-effective way. This report discusses the current state-of-the-art as well as future developments in GMO detection.
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J Vis
January 2025
Neural Information Processing Group, University of Tübingen, Tübingen, Germany.
Human performance in psychophysical detection and discrimination tasks is limited by inner noise. It is unclear to what extent this inner noise arises from early noise (e.g.
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December 2024
Grain Research Laboratory, Canadian Grain Commission, Winnipeg, MB R3P1N1, Canada.
The number of genetically modified (GMO) events for canola, corn, and soybean is steadily increasing. Some countries, including those in the EU, have regulatory requirements for the approval and use of plant ingredients containing GMOs. Multiplex digital PCR (dPCR) has been used for the simultaneous detection and quantification of various GMO events.
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December 2024
National Reference Laboratory for GM Food and Feed, GMO Unit, Istituto Zooprofilattico Sperimentale del Lazio e della Toscana "Mariano Aleandri", 00178 Rome, Italy.
Due to the increasing number of authorized events in the European Union, it is crucial for the official laboratories to enforce market control to detect and quantify genetically modified organisms. In this study, an in-house validation of quantitative duplex ddPCR methods was performed involving MON87701, MON87769, MON89788 and CV-127-9 assays with respect to the lectin reference gene. Since the ddPCR methods provide accurate quantification, show less sensitivity to PCR inhibitors and are more suitable for multiplexing compared to the real-time PCR, the optimization of the existing assays was performed with the exception of MON87701, according to the JRC Guidance documents and technical reports.
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November 2024
Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, College of Life Sciences, China Jiliang University, Hangzhou, Zhejiang, China.
With the rapid development of transgenic technology and the increasing prevalence of genetically modified (GMO) crops, incidents such as illegal importation, environmental contamination, and safety concerns associated with GMOs have risen significantly in recent years. Consequently, there is a growing demand for more advanced methods of GMO crop detection. Traditional molecular detection techniques, which rely on nucleic acids or proteins, are becoming less effective due to the increasing complexity of GMO crop genomes.
View Article and Find Full Text PDFGM Crops Food
December 2024
Department Method Standardisation, Reference Laboratories, Resistance to Antibiotics, Federal Office of Consumer Protection and Food Safety (BVL), Berlin, Germany.
Under current EU legislation, genetically modified organisms (GMOs) and derived food and feed products must be authorized as GM food, feed, or seed and appropriate detection methods must be made available for use in official controls. A Real-Time PCR method has recently been published by Chhalliyil et al. claiming to be specific for the detection and identification of genome-edited oilseed rape (OSR) lines commercialized in North America.
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