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Emerging phospholipid-targeted affinity materials for extracellular vesicle isolation and molecular profiling.
J Chromatogr A
December 2024
Beijing National Laboratory for Molecular Sciences, CAS Key Laboratory of Analytical Chemistry for Living Biosystems, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China; School of Chemistry, University of Chinese Academy of Sciences, Beijing, 100049, China. Electronic address:
Extracellular vesicles (EVs) carrying lipids, proteins, nucleic acids and small molecular metabolites have emerged as an attractive paradigm for understanding and interfering physiological and pathological processes. To this end, selective and efficient separation approaches are highly demanded to obtain target EVs from complicated biosamples. With increasing knowledges on EV lipids, recent years have witnessed rapid advances of phospholipid-targeted affinity materials and platforms for high-performance isolation and analysis of EVs.
View Article and Find Full Text PDFBioinformatics identification based on causal association inference using multi-omics reveals the underlying mechanism of Gui-Zhi-Shao-Yao-Zhi-Mu decoction in modulating rheumatoid arthritis.
Phytomedicine
December 2024
Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu 610072, China. Electronic address:
Object: Rheumatoid arthritis (RA) is a prevalent and currently incurable autoimmune disease. Existing conventional medical treatments are limited in their efficacy, prolonged disease may lead to bone destruction, joint deformity, and loss of related functions, which places a huge burden on RA patients and their families. For millennia, the use of traditional Chinese medicine (TCM), exemplified by the Gui-Zhi-Shao-Yao-Zhi-Mu decoction (GZSYZM), has been demonstrated to offer distinct therapeutic advantages in the management of RA.
View Article and Find Full Text PDFConstruction of amino functional metal-organic framework modified aramid composite separators with high Li transport channels for dendrite-free lithium-ion batteries.
J Colloid Interface Sci
December 2024
School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin 300400 PR China. Electronic address:
The formation and growth of lithium dendrites is an ever-present and urgent problem in lithium-ion batteries (LIBs). At the same time, the low melting point of commercial polyolefin separators may lead to safety issues during application. On this basis, in this work, poly (m-phenylene isophthalamide) (PMIA)/Zr-based metal-organic framework (NH-UiO-66) composite separator was prepared by non-solvent induced phase separation (NIPS).
View Article and Find Full Text PDFNature's arsenal unleashed: derived thymol halts cancer progression by suppressing proangiogenic genes.
Nat Prod Res
December 2024
Department of Zoology, GC University, Lahore, Pakistan.
Inhibiting angiogenesis with plant-derived bioactive compounds can inhibit tumour progression. Antiangiogenic potential of was analysed by preparing and analysing ethanolic extracts of by GC-MS and HPLC to identify bioactive components. In-vivo blood vessel formation assays in mice and chorioallantoic membrane assays (CAM) in eggs were employed to assess the antiangiogenic effects.
View Article and Find Full Text PDFDevelopment of high-performance inducible and secretory expression vector and host system for enhanced recombinant protein production.
Sci Rep
December 2024
Department of Comparative Biomedical Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi state, MS, 39762, USA.
The production of lipopolysaccharide (LPS)-free recombinant proteins from culture supernatants is of great interest to biomedical research and industry. Due to the LPS-free cell wall structure and the well-defined secretion factor B (SecB)-dependent secretion pathway, Gram-positive bacteria are a superior alternative to Escherichia coli expression systems. However, the lack of inducible expression systems for high yields has been a bottleneck.
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