The genomic cloning and sequence of hepatitis E virus (HEV) from an epidemic in North India is reported. We describe here a simple method wherein the viral RNA was reverse transcribed and then amplified in a single step using an extra long polymerase chain reaction procedure. The full genome nucleotide sequence of this HEV isolate (called Yam-67) was made up of 7191 nucleotides, excepting the poly(A) tail and had three open reading frames: ORF1 coding for 1693 amino acids (aa), ORF2 coding for 659 aa and ORF3 coding for 122 aa. This North Indian isolate of HEV showed close sequence homology to other HEV isolates from India and Asia, but was distant from the Chinese genotype 4, Japanese, Mexican and US isolates. There is no indication from sequence analysis that this may be an atypical strain of HEV, as reported earlier.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/s0168-1702(02)00052-7 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
ALS: A Silent Slayer of Motor Neurons. Traditional Chinese Herbal Medicine as an Effective Therapy.
Curr Pharm Des
January 2025
Amity Institute of Pharmacy, Lucknow, Amity University, Uttar Pradesh, India.
Amyotrophic Lateral Sclerosis (ALS), is a progressive neurodegenerative disease characterized by motor symptoms, and cognitive impairment. The complexity in treating ALS arises from genetic and environmental factors, contributing to the gradual decline of lower and upper motor neurons. The anticipated pharmaceutical market valuation for ALS is projected to reach $1,038.
View Article and Find Full Text PDFA Pilot Study of the Anthropometric Growth Pattern of the Human Auricle of the North Indian Region.
Cureus
December 2024
Department of Otolaryngology, Head and Neck Surgery, Wrightington, Wigan and Leigh NHS Foundation Trust, Wigan, GBR.
Objectives To assess the dimensions of external ear (pinna) in different age groups in the North Indian population. To assess the mean dimensions of external ear (pinna) in different age groups in North Indian males and females. Methods The study area was Lucknow/Barabanki, Uttar Pradesh, and the study center was Era's Lucknow Medical College, Uttar Pradesh, India.
View Article and Find Full Text PDFElastographic evaluation for fatty liver disease in north Indian children and adolescents with type 1 diabetes.
J Pediatr Endocrinol Metab
January 2025
Pediatrics, Postgraduate Institute of Medical Education & Research, Chandigarh, India.
Objectives: The prevalence and predisposing factors to metabolic dysfunction-associated fatty liver disease (MAFLD) in children with type 1 Diabetes (T1D) living in developing countries are unknown.
Methods: A cross-sectional study was conducted in children with T1D. The presence of liver fat and tissue stiffness were assessed by ultrasonography and shear-wave elastography (SWE), respectively.
Genomics-assisted stacking of waxy1, opaque2, and crtRB1 genes for enhancing amylopectin in biofortified maize for industrial utilization and nutritional security.
Funct Integr Genomics
January 2025
ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India.
Waxy maize is highly preferred diet in developing countries due to its high amylopectin content. Enriching amylopectin in biofortified maize meets food security and fulfils the demand of rising industrial applications, especially bioethanol. The mutant waxy1 (wx1) gene is responsible for increased amylopectin in maize starch, with a wide range of food and industrial applications.
View Article and Find Full Text PDFEvaluation of Predictors of Response to Ovulation Induction Using Letrozole in Women with Polycystic Ovary Syndrome: A Prospective Cohort Study.
J Hum Reprod Sci
December 2024
Department of Obstetrics and Gynaecology, All India Institute of Medical Sciences, New Delhi, India.
Background: Ovulation induction (OI) in patients with polycystic ovary syndrome (PCOS) remains challenging, and several biomarkers have been evaluated for their ability to predict ovulation. The predictive ability of candidate biomarkers, particularly with letrozole-based therapy in infertile PCOS women, remains inconclusive as it is yet to be evaluated in a prospective study.
Aim: To assess the role of anti-Müllerian hormone (AMH), follicle-stimulating hormone (FSH), luteinising hormone (LH)/FSH ratio, testosterone and free androgen index (FAI) as predictors of ovarian response to letrozole-based OI therapy during OI cycles in infertile women with PCOS from North India.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!