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Metasurface Plasmon Resonance Biosensor Enhanced with Dual Gold Nanoparticles for the Ultrasensitive Quantitative Detection of C-Reactive Protein.
Nano Lett
January 2025
College of Life Science and Technology, State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Huazhong University of Science and Technology, Wuhan 430074, China.
The pursuit of cutting-edge diagnostic systems capable of detecting biomarkers with exceptional sensitivity and precision is crucial for the timely and accurate monitoring of inflammatory responses. In this study, we introduce a dual gold nanoparticle-enhanced metasurface plasmon resonance (Bi-MSPR) biosensor for the ultrasensitive detection of C-reactive protein (CRP). The Bi-MSPR sensor is constructed upon a nanocup array chip with gradient-free electron density, where an innovative metasurface structure is built using a PEI-immobilized dual-gold nanoparticle amplification system.
View Article and Find Full Text PDFHeat stress promotes osteogenic and odontogenic differentiation of stem cells from apical papilla via glucose-regulated protein 78-mediated autophagy.
J Dent Sci
January 2025
Endodontic Department, Changzhou Stomatological Hospital, Changzhou, China.
Background/purpose: Heat stress is essential for improving the efficacy of mesenchymal stem cell (MSC)-based regeneration medicine. However, it is still unclear whether and how heat stress influences the differentiation of stem cells from apical papilla (SCAPs). This research aimed to explore the potential mechanism of glucose-regulated protein 78 (GRP78) in regulating differentiation under heat stress in SCAPs.
View Article and Find Full Text PDFProtein synthesis is by far the most energetically costly cellular process in rapidly dividing cells. Quantifying translating ribosomes in individual cells and their average mRNA transit rate is arduous. Quantitating assembled ribosomes in individual cells requires electron microscopy and does not indicate ribosome translation status.
View Article and Find Full Text PDFBackground: Atherosclerosis (AS) is caused by the endothelium injury associated with oxidative stress. Previous studies have shown that the Phlegm-Eliminating and Stasis- Transforming Decoction (Huayu Qutan Decoction, HYQTD) has mitochondrial protective function. The objective of this research was to explore how HYQTD drug-containing serum (HYQTD-DS) could potentially protect mitochondrial energy production in endothelial cells (ECs) from injury caused by hydrogen peroxide (H2O2)-induced oxidative damage in AS through SIRT1/PGC-1α/ Nrf2 pathway.
View Article and Find Full Text PDFHigh-Efficiency CRISPR-Cas9 Genome Editing Unveils Biofilm Insights and Enhances Antimicrobial Activity in Bacillus velezensis FZB42.
Biotechnol Bioeng
January 2025
Department of Biosystems Engineering, Auburn University, Auburn, Alabama, USA.
Bacillus velezensis FZB42 is a prominent plant growth-promoting rhizobacterium and biocontrol agent known for producing a wide array of antimicrobial compounds. The capability to genetically manipulate this strain would facilitate understanding its metabolism and enhancing its sustainable agriculture applications. In this study, we report the first successful implementation of high-efficiency CRISPR-Cas9 genome editing in B.
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