AI Article Synopsis
- P-TEFb, comprising CDK9 and cyclin T(1), was purified from a cell line with epitope-tagged CDK9, revealing proteins like cyclin T isoforms, HSP90, CDC37, and a novel protein named MCEF.
- MCEF is a newly discovered member of the AF4 transcription factor family, which is associated with acute lymphoblastic leukemia, and its RNA is found in all human tissues studied.
- Despite being associated with P-TEFb, MCEF does not activate HIV-1 replication or transcription when tethered to a promoter.
Article Abstract
Positive transcription elongation factor-b (P-TEFb) contains CDK9 and cyclin T(1). P-TEFb was affinity purified from a stably transfected cell line that expresses epitope-tagged CDK9, and proteins that appeared to be specifically bound were sequenced. In addition to CDK9, previously identified isoforms of cyclin T (including T(1), T(2A) and T(2B)), HSP90 and CDC37, this analysis identified a novel protein named MCEF. Cloning of its cognate cDNA revealed that MCEF is the newest member of the AF4 family of transcription factors involved in acute lymphoblastic leukemia. MCEF RNA was expressed in all human tissues examined, and antisera directed against recombinant MCEF specifically immunoprecipitated P-TEFb. Ectopic expression of MCEF did not activate HIV-1 replication, and tethering of MCEF to a promoter did not activate transcription.
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| http://dx.doi.org/10.1007/BF02256070 | DOI Listing |
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MCEF, the newest member of the AF4 family of transcription factors involved in leukemia, is a positive transcription elongation factor-b-associated protein.
J Biomed Sci
March 2003
Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, New York, N.Y., USA.
Article Synopsis
- P-TEFb, comprising CDK9 and cyclin T(1), was purified from a cell line with epitope-tagged CDK9, revealing proteins like cyclin T isoforms, HSP90, CDC37, and a novel protein named MCEF.
- MCEF is a newly discovered member of the AF4 transcription factor family, which is associated with acute lymphoblastic leukemia, and its RNA is found in all human tissues studied.
- Despite being associated with P-TEFb, MCEF does not activate HIV-1 replication or transcription when tethered to a promoter.
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