AI Article Synopsis
- A novel capillary zone electrophoresis method was developed for quick analysis of nucleotides, achieving high resolution in under 5 minutes.
- The technique involved using a specific voltage and phosphate buffer, with detection limits for key nucleotides (ATP, UDP, UTP) between 0.14 and 0.28 microM.
- This method helped assess the purity of nucleotides for pharmacological studies and was also used to investigate nucleotide metabolism in a specific cell line, as well as enzymatic activity involving hexokinase.
Article Abstract
An easy and fast method for the quantitative analysis of nucleotides by capillary zone electrophoresis was developed. The method employing a neutral-bonded capillary and reversed polarity mode provided a good resolution and a short analysis time of less than 5 min. The samples were injected electrokinetically using -6 kV voltage for 30 s and detected by their UV absorbance at 254 nm. Constant current (-45 microA) was applied, and a phosphate buffer, pH 7.4, was used. The detection limits for ATP, UDP, and UTP ranged between 0.14 and 0.28 microM. This method was required for the investigation of the purity of the commercially available nucleotides used in pharmacological studies. In addition, the analytical method was applied to study the metabolism of nucleotides in a cell line, neuroblastoma x glioma hybrid cells (NG108-15), which is used in pharmacological studies with nucleotides, since it contains purine- and pyrimidine-sensitive nucleotide receptors. Furthermore, we used the new method for monitoring enzymatic studies using the enzyme hexokinase to convert nucleotide triphosphates to diphosphates.
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| http://dx.doi.org/10.1016/s0021-9673(02)00095-x | DOI Listing |
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