Stress-induced changes of plasma antioxidants in aquacultured sea bass, Dicentrarchus labrax.

Comp Biochem Physiol A Mol Integr Physiol

Department of Zoology, Federico II University of Naples, Via Mezzocannone 8, 80134 Naples, Italy.

Published: May 2002

Antioxidant plasma activities of ascorbate, alpha-tocopherol and glutathione peroxidase were analysed in adult male sea bass, Dicentrarchus labrax, in normal conditions and after hypoxia-recovery. In addition, tank measurements of temperature, pH, salinity and chlorine changes were carried out. Ascorbate and alpha-tocopherol were measured using a high-pressure liquid chromatography method and glutathione peroxidase activity enzymatically. Ascorbate and alpha-tocopherol showed a relationship with the velocity of body growing in normal and hypoxia-recovery conditions. In sea bass exposed to hypoxia, only ascorbate and alpha-tocopherol levels were significantly lower compared with the control group. Slope study and expression percent of antioxidants reduction after stress conditions revealed a predominant role of plasma alpha-tocopherol. Sea bass subjected to variations of salinity and chlorine showed a significant decrease in plasma alpha-tocopherol. A relationship could be suggested between antioxidant defence and fish response in aquaculture.

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http://dx.doi.org/10.1016/s1095-6433(01)00549-9DOI Listing

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