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Rapid Multi-Well Evaluation of Assorted Materials for Hydrogel-Assisted Giant Unilamellar Vesicle Production: Empowering Bottom-Up Synthetic Biology.
Gels
January 2025
Institute of Synthetic Bioarchitectures, Department of Bionanosciences, University of Natural Resources and Life Sciences, Vienna, Muthgasse 11, Level 2, 1190 Vienna, Austria.
Giant unilamellar vesicles (GUVs) are versatile cell models in biomedical and environmental research. Of the various GUV production methods, hydrogel-assisted GUV production is most easily implemented in a typical biological laboratory. To date, agarose, polyvinyl alcohol, cross-linked dextran-PEG, polyacrylamide, and starch hydrogels have been used to produce GUVs.
View Article and Find Full Text PDFInfluence of Internal Architecture and Ink Formulation on the Thermal Behavior of 3D-Printed Cementitious Materials.
Materials (Basel)
November 2024
Department of Civil and Environmental Engineering, Vanderbilt University, PMB 351831, 2301 Vanderbilt Place, Nashville, TN 37235-1831, USA.
Cement-based 3D printing provides an opportunity to create cement-based elements with a hierarchy of structures and patterns that are not easily achievable using traditional casting techniques, thereby providing new possibilities for improving thermal control and energy storage in cement-based materials. In this study, the influence of internal architecture and ink formulation on the thermal behavior of 3D-printed cement composite beams was investigated using infrared thermal imaging and a conceptual one-dimensional heat transfer model based on cooling fins in convective media. Three-dimensional printed beams with rectilinear, three-dimensional honeycomb, and Archimedean chord infill patterns and cement ink formulations with and without 5% halloysite nanoclay were exposed to a heating source at one end.
View Article and Find Full Text PDFExperimental determination of the mechanical and hydrolytic properties of chitosan/rice husk ash composite membranes.
Int J Biol Macromol
January 2025
Department of Materials Science and Engineering, African University of Science and Technology, Abuja FCT 900107, Nigeria. Electronic address:
Chitosan-based membranes are promising alternatives to synthetic membranes in a number of specialized use cases, including water purification and electrochemical devices. In application, excessive swelling when hydrated can lead to poor mechanical integrity, necessitating modifications to the polymer so as to counter this effect. Embedding inorganic fillers within an organic polymer matrix is one method of combining excellent mechanical stability with good performance.
View Article and Find Full Text PDFThe promise of CRISPR-associated transposons for bacterial functional genomics.
Curr Opin Microbiol
December 2024
Pharmaceutical Sciences Division, School of Pharmacy, University of Wisconsin-Madison, Madison, WI 53705, USA; Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, WI 53726, USA; Department of Bacteriology, University of Wisconsin-Madison, Madison, WI 53706, USA; Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, WI 53706, USA; Center for Genomic Science Innovation, University of Wisconsin-Madison, Madison, WI 53706, USA. Electronic address:
CRISPR-associated transposons (CASTs) are naturally occurring amalgamations of CRISPR-Cas machinery and Tn7-like transposons that direct site-specific integration of transposon DNA via programmable guide RNAs. Although the mechanisms of CAST-based transposition have been well studied at the molecular and structural level, CASTs have yet to be broadly applied to bacterial genome engineering and systematic gene phenotyping (i.e.
View Article and Find Full Text PDFTreatment of scoliosis in children after resection of neuroblastoma-A report of five cases.
J Child Orthop
December 2024
Department of Orthopaedics, Beijing Children's Hospital, National Center for Children's Health, Beijing, China.
Article Synopsis
- * A study evaluated five patients who developed spinal deformities post-NB resection, using techniques like growing rods, spinal fusion, and casting, achieving significant improvement in their conditions.
- * It's recommended to monitor spinal deformities closely and start treatment within 6 months of diagnosis, tailoring the approach based on the patient's growth potential and skeletal maturity.
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