AI Article Synopsis
- The study focuses on parallel-stranded hairpin structures containing a polypyrimidine sequence linked to complementary purines, specifically 8-aminopurines.
- Stability of these triplex formations was evaluated through UV-absorption melting experiments under varying pH and salt concentrations, showing they remain stable even at neutral pH.
- Techniques like gel-shift experiments, circular dichroism, and NMR spectroscopy confirmed the robustness of these modified hairpins, suggesting they can be adapted to address minor gaps in the target polypyrimidine sequences.
Article Abstract
Parallel-stranded hairpins with a polypyrimidine sequence linked to a complementary purine carrying 8-aminopurines such as 8-aminoadenine, 8-aminoguanine and 8-aminohypoxanthine bind polypyrimidine sequences complementary (in an antiparallel sense) to the purine part by a triple helix. The relative stabilities of triplexes were assessed by UV-absorption melting experiments as a function of pH and salt concentration. Hairpins carrying 8-aminopurines give very stable triple helical structures even at neutral pH, as confirmed by gel-shift experiments, circular dichroism and nuclear magnetic resonance spectroscopy. The modified hairpins may be redesigned to cope with small interruptions in the polypyrimidine target sequence.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC117286 | PMC |
| http://dx.doi.org/10.1093/nar/gkf374 | DOI Listing |
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