The barotolerant E.coli TG1P, DH5alphaP, HB101P were obtained from TG1,DH5alpha, HB101 after treatment with high hydrostatic pressure, and the survival rates of the formers raised 2--3 magnitude, from 1.06x10(-6), 2.98x10(-4), 3.65x10(-3) to 8.31x10(-3), 3.40x10(-1), 1.69x10(-1),respectively. Comparison of partial proteomes of wild and barotolerant bacteria of TG1 and DH5alpha with two-dimensional electrophoresis showed that three proteins were obviously highly expressed in barotolerant bacteria. The N terminal sequence and molecular weight of one protein was shown to be V(L)EAGEFFMRA and 21 kD, with high identity to a known outmembrane protein in E.coli.
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