Using the pig splenic nerve as a model, we investigated the proteolytic processing of porcine chromogranin B (CgB) during its axonal transport. An ELISA was developed for SR-17 (CgB(586-602)), a novel CgB-derived peptide, originally found in the adrenal medulla. The results demonstrate that CgB is processed in an early stage during its axonal transport. Immunohistochemical data, based on a rabbit anti-SR-17 antiserum, show that the spleen CgB/SR-17 is exclusively present in the nerve endings. No SR-17 immunoreactivity (IR) was found in splenocytes. We also provide evidence that SR-17 is co-released with noradrenaline (NA) upon electrical stimulation of the splenic nerve. Its release is frequency-dependent and strongly enhanced in the presence of the alpha-blocking agent phentolamine. In addition, we show that the new CgB-peptide can serve as a substrate for the lymphocyte surface glycoprotein CD26, also known as dipeptidyl peptidase IV (DPP IV), generating a new peptide ER-15 (CgB(588-602)).
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/s0167-0115(02)00038-1 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
ProBDNF as a Myokine in Skeletal Muscle Injury: Role in Inflammation and Potential for Therapeutic Modulation of p75.
Int J Mol Sci
January 2025
Division of Basic Biomedical Sciences, University of South Dakota Sanford School of Medicine, Vermillion, SD 57069, USA.
Brain-derived neurotropic factor (BDNF) is expressed by skeletal muscle as a myokine. Our previous work showed that the active precursor, proBDNF, is the predominant form of BDNF expressed in skeletal muscle, and that following skeletal muscle injury, proBDNF levels are significantly increased. However, the function of the muscle-derived proBDNF in injury-induced inflammation has yet to be fully understood.
View Article and Find Full Text PDFThe spleen in ischaemic heart disease.
Nat Rev Cardiol
January 2025
Institute for Pathophysiology, West German Heart and Vascular Center, University of Duisburg-Essen, Essen, Germany.
Article Synopsis
- Ischaemic heart disease results from coronary atherosclerosis, which is linked to systemic inflammation involving various immune cells released by the spleen.
- Prolonged inflammation can lead to ischaemic heart failure, while the spleen's interaction with the nervous system can modulate immune responses and protect the heart from damage.
- Splenectomy, which removes the spleen, increases mortality risk from ischaemic heart disease, highlighting the spleen's crucial role in immune responses and cardiovascular protection.
Left T7 paravertebral nerve blockade activate the α7nAChR-Dependent CAP in patients undergoing thoracoscopic lobectomy: a prospective controlled study.
BMC Anesthesiol
December 2024
Department of Anesthesiology and Surgery, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, Jiangxi, 330001, China.
Objective: This study aimed to observe the impact of Tthoracic paravertebral nerve blockade(TPVB) at left T7 level on the α7nAChR-dependent cholinergic anti-inflammatory pathway in patients undergoing thoracoscopic lobectomy.
Methods: Scheduled thoracoscopic lung surgery patients at the First Affiliated Hospital of Nanchang University from August to September 2023 were divided into two groups according to the surgical site. The experimental group underwent left T7 paravertebral nerve blockade (LTPVB group), while the control group underwent right T7 paravertebral nerve blockade (RTPVB group).
Klippel-Trenaunay syndrome and chiari I malformation. A case report and systematic review of the literature.
Brain Spine
November 2024
Neurosurgical Department, "KAT" General Hospital of Athens, Greece.
Introduction: Klippel-Trenaunay Syndrome (KTS) is a rare congenital condition characterized by vascular malformations, bone abnormalities, and limb overgrowth. The genetic basis of KTS is not fully understood, and the diagnosis relies on clinical features. Its clinical spectrum includes several neurosurgical diagnoses, such as cavernous hemangiomas, arteriovenous fistulas, and Chiari I malformation.
View Article and Find Full Text PDF[Scientific connotation of Zangfu theory in traditional Chinese medicine from modern biological research on "organ crosstalk"].
Zhongguo Zhong Yao Za Zhi
November 2024
Center for Evidence-based Chinese Medicine, Institute of Basic Research in Clinical Medicine,China Academy of Chinese Medical Sciences Beijing 100700, China.
Life is a dynamic and systematic whole, with organs interacting with each other. Traditional Chinese medicine(TCM) discusses the interaction between human tissue and organs through the Zangfu relations, while modern biological medicine clarifies physiological and pathological relationships through "organ crosstalk". From the perspective of "organ crosstalk", this article discussed the five classical Zangfu relations(lungs and large intestine, heart and small intestine, spleen and stomach, liver and gallbladder, and kidney and bladder) in TCM and their extension.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!