Recently an immunoblotting system was developed for typing oxacillin-resistant Staphylococcus aureus. Oxacillin-resistant S. aureus (ORSA) isolates recovered during a 4-month period from premature new-borns division of University Hospital of Pavia, with several antimicrobial susceptibility patterns, were evaluated with this typing scheme. Immunoblotting was found to be a useful method with good reproducibility and the ORSA strains isolated at our institution were differentiated in two major groups of organisms clinically and epidemiologically related. Furthermore immunoblot typing showed same specific correlations with susceptibility patterns of ORSA isolates studied.
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Strain-level genomic analysis of serotype, genotype and virulence gene composition of group B streptococcus.
Front Cell Infect Microbiol
November 2024
Department of Obstetrics and Gynecology, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua University, Beijing, China.
Introduction: GBS (group B streptococcus) is an opportunistic pathogen that can colonize healthy individuals but presents significant challenges in clinical obstetrics and gynecology, as it can cause miscarriage, preterm birth, and invasive infections in newborns. To develop specific and personalized preventative strategies, a better understanding of the epidemiological characteristics and pathogenic features of GBS is essential.
Methods: We conducted a comprehensive strain-level genomic analysis of GBS, examining serotype and genotype distributions, as well as the composition and correlations of virulence genes using the blastn-short mode of the BLAST program(v2.
Protocol for the neonatal intracerebroventricular delivery of adeno-associated viral vectors for brain restoration of MECP2 for Rett syndrome.
STAR Protoc
December 2024
Department of Developmental and Behavioural Pediatric & Child Primary Care, Brain and Behavioural Research Unit of Shanghai Institute for Pediatric Research and MOE-Shanghai Key Laboratory for Children's Environmental Health of Xinhua Hospital & Songjiang Research Institute, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China. Electronic address:
Article Synopsis
- The protocol provides a method for delivering adeno-associated viral vectors (AAVs) into the brains of neonatal mice as a gene therapy for Rett syndrome.
- It outlines the necessary steps for preparing the mouse models, including mouse line preparation, fostering, sex typing, and genotyping.
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Changes in Physiopathological Markers in Myotonic Dystrophy Type 1 Skeletal Muscle: A 3-Year Follow-up Study.
J Neuromuscul Dis
September 2024
Groupe de Recherche Interdisciplinaire sur les Maladies Neuromusculaires (GRIMN), Centre Intégré Universitaire de Santé et de Services Sociaux du Saguenay-Lac-Saint-Jean, Saguenay, QC, Canada.
Article Synopsis
- - Myotonic dystrophy type 1 (DM1) is a disease caused by abnormal CTG repeats in a specific gene, leading to muscle weakness and wasting, with limited research on how it affects skeletal muscle over time.
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Factors associated with non-pathogenic antibodies against desmoglein-3 in pemphigus foliaceus.
An Bras Dermatol
August 2024
Laboratory of the Dermatology Division, Hospital das Clínicas, Faculty of Medicine of Ribeirão Preto, Universidade de São Paulo, São Paulo, SP, Brazil; Division of Dermatology, Department of Internal Medicine, Faculty of Medicine of Ribeirão Preto, Universidade de São Paulo, São Paulo, SP, Brazil. Electronic address:
Background: Anti-desmoglein (Dsg)1 is produced in pemphigus foliaceus (PF), affecting exclusively the skin. Pemphigus vulgaris (PV) shows the production of anti-Dsg3 in the mucosal form, and anti-Dsg1 and 3 in the mucocutaneous form. Anti-Dsg3 autoantibodies have been rarely reported in PF.
View Article and Find Full Text PDFClassical BSE dismissed as the cause of CWD in Norwegian red deer despite strain similarities between both prion agents.
Vet Res
May 2024
Centro de Investigación en Sanidad Animal (CISA), Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Consejo Superior de Investigaciones Científicas (CSIC), Madrid, Spain.
The first case of CWD in a Norwegian red deer was detected by a routine ELISA test and confirmed by western blotting and immunohistochemistry in the brain stem of the animal. Two different western blotting tests were conducted independently in two different laboratories, showing that the red deer glycoprofile was different from the Norwegian CWD reindeer and CWD moose and from North American CWD. The isolate showed nevertheless features similar to the classical BSE (BSE-C) strain.
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