pDOR-erbB-2 sense and antisense retroviral expression vectors were introduced into normal human diploid fibroblasts(2BS), respectively, to construct 2BS-A cells(transfected with antisense recombinant vector) and 2BS-S cells (transfected with sense recombinant vector). Southern blot analysis verified that the exgenous c-erbB-2 cDNA were integrated into genomic DNA. Compared with the cells transfected with pDOR-neo empty vector, 2BS-A cells exhibited decreased ability not only to repair DNA damage, but also to undergo apoptosis. This was consistent with our observations that senescent phenotype was earlier appeared in these cells.
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