AI Article Synopsis
- Researchers tested different thymidine analogues to see if they could induce a senescence-like state in HeLa cells.
- CldU, BrdU, and IdU caused changes typical of senescent cells, while other analogues primarily resulted in cell death.
- Among the tested compounds, 5-halogenated thymidine analogues were the only ones that specifically triggered cellular senescence in mammalian cells.
Article Abstract
We tested various thymidine analogues for induction of a senescence-like phenomenon in HeLa cells. CldU, BrdU, and IdU similarly induced the morphology of senescent cells and typical senescence markers. Thymidine analogues other than 5-halogenated forms caused only cell death. BrdU efficiently killed the cells in cooperation with irradiation with light and a brief treatment with Hoechst 33258, but CldU did not at all. 5-Halogenated thymidine analogues were thus shown to be specific inducers of cellular senescence in mammalian cells.
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| http://dx.doi.org/10.1271/bbb.66.877 | DOI Listing |
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